PRODUCT CODE: ET1704-50

Recombinant Clathrin heavy chain Monoclonal Antibody (ET1704-50)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Clathrin heavy chain on Hela (1) and PC-12 (2) cell lysate using anti-Clathrin heavy chain antibody at 1/1,000 dilution.
  • Western blot analysis of Clathrin heavy chain on Hela (1) and PC-12 (2) cell lysate using anti-Clathrin heavy chain antibody at 1/1,000 dilution.
Western blot analysis of Clathrin heavy chain on Hela (1) and PC-12 (2) cell lysate using anti-Clathrin heavy chain antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Clathrin heavy chain Monoclonal Antibody (ET1704-50)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, PC12, human adrenal gland tissue, human colon cancer tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA53-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

191/187 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Clathrin heavy chain 2

GENE NAME

CLTCL1

SYNONYMS

CLH-22, CLTCL1, CLH22, CLTCL, CLTD

SEQUENCE SIMILARITIES

Belongs to the clathrin heavy chain family.

TISSUE SPECIFICITY

Maximal levels in skeletal muscle. High levels in heart and testis. Low expression detected in all other tissues.

SUBCELLULAR LOCATION

Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic face of coated pits and vesicles.

FUNCTION

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network (By similarity).