PRODUCT CODE: ET1703-08

Recombinant Chromogranin A Monoclonal Antibody (ET1703-08)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of Chromogranin A on PC-12 cells lysates using anti-Chromogranin A antibody at 1/1,000 dilution.
  • Western blot analysis of Chromogranin A on PC-12 cells lysates using anti-Chromogranin A antibody at 1/1,000 dilution.
  • ICC staining Chromogranin A in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Chromogranin A antibody. Counter stained with hematoxylin.
Western blot analysis of Chromogranin A on PC-12 cells lysates using anti-Chromogranin A antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Chromogranin A Monoclonal Antibody (ET1703-08)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-12, PC-3M, human pancreas tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM103-7

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

86 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Chromogranin-A

GENE NAME

CHGA

SYNONYMS

CgA, SP-I, CHGA

SEQUENCE SIMILARITIES

Belongs to the chromogranin/secretogranin protein family.

TISSUE SPECIFICITY

GE-25 is found in the brain.

POST-TRANSLATIONAL MODIFICATION

Sulfated on tyrosine residues and/or contains sulfated glycans.; O-glycosylated with core 1 or possibly core 8 glycans.; Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity. Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides.

SUBCELLULAR LOCATION

[Serpinin]: Secreted. Cytoplasmic vesicle, secretory vesicle. Note=Pyroglutaminated serpinin localizes to secretory vesicle.; Cytoplasmic vesicle, secretory vesicle. Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle. Secreted. Note=Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions. In pituitary gonadotropes, located in large secretory granules.

FUNCTION

[Pancreastatin]: Strongly inhibits glucose induced insulin release from the pancreas.; [Catestatin]: Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist. Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa. Can induce mast cell migration, degranulation and production of cytokines and chemokines. Acts as a potent scavenger of free radicals in vitro. May play a role in the regulation of cardiac function and blood pressure.; [Serpinin]: Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.