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PRODUCT CODE: ET7106-90

Recombinant Cellular Apoptosis Susceptibility Monoclonal Antibody (ET7106-90)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Cellular Apoptosis Susceptibility on different lysates using anti-Cellular Apoptosis Susceptibility antibody at 1/500 dilution.<br /> Positive control:<br /> Lane 1: Mouse testis<br /> Lane 2: SiHa<br /> Lane 3: SK-BR-3<br /> Lane 4: PC-3M
  • Western blot analysis of Cellular Apoptosis Susceptibility on different lysates using anti-Cellular Apoptosis Susceptibility antibody at 1/500 dilution.<br /> Positive control:<br /> Lane 1: Mouse testis<br /> Lane 2: SiHa<br /> Lane 3: SK-BR-3<br /> Lane 4: PC-3M
  • ICC staining Cellular Apoptosis Susceptibility in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Cellular Apoptosis Susceptibility in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Cellular Apoptosis Susceptibility in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cellular Apoptosis Susceptibility antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cellular Apoptosis Susceptibility antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of LOVO cells with Cellular Apoptosis Susceptibility antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Cellular Apoptosis Susceptibility on different lysates using anti-Cellular Apoptosis Susceptibility antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse testis
Lane 2: SiHa
Lane 3: SK-BR-3
Lane 4: PC-3M

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cellular Apoptosis Susceptibility Monoclonal Antibody (ET7106-90)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse testis tissue, SiHa, SK-BR-3, PC-3M, LOVO, SH-SY5Y, human colon cancer tissue, LOVO.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU34-33

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

110 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P55060

PROTEIN NAME

Cellular Apoptosis Susceptibility

SYNONYMS

CAS antibody; Cellular apoptosis susceptibility protein antibody; Chromosome segregation 1 (yeast homolog) like antibody; Chromosome segregation 1 Like antibody; Chromosome segregation 1 like protein antibody; Chromosome segregation 1-like protein antibody; Chromosome segregation gene CSE1 antibody; CSE 1 antibody; CSE 1 chromosome segregation 1 like antibody; CSE 1 chromosome segregation 1 like protein antibody; CSE 1L antibody; CSE1 antibody; CSE1 chromosome segregation 1 like (yeast) antibody; CSE1 chromosome segregation 1 like antibody; CSE1 chromosome segregation 1 like protein antibody; CSE1L antibody; Exp 2 antibody; Exp2 antibody; Exportin 2 antibody; Exportin-2 antibody; Exportin2 antibody; Importin alpha re exporter antibody; Importin-alpha re-exporter antibody; MGC117283 antibody; MGC130036 antibody; MGC130037 antibody; XPO 2 antibody; XPO2 antibody; XPO2_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the XPO2/CSE1 family.

TISSUE SPECIFICITY

Detected in brain, placenta, ovary, testis and trachea (at protein level). Widely expressed. Highly expressed in testis and in proliferating cells.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Cellular apoptosis susceptibility protein (CAS), also called Exportin 2, is a 971 amino acid member of the CSE1 family. CAS mediates Importin α re-export from the nucleus to the cytoplasm after import substrates have been released into the nucleoplasm. In the nucleus, CAS binds cooperatively to Importin α and to the GTPase Ran in its GTP-bound (active) form. This complex binds to nucleoporins as it docks to the nuclear pore complex. Once in the cytoplasm, the complex dissociates and Importin α is released and CAS returns to the nuclear compartment and the process begins anew. CAS can be detected highly in proliferating cells. Three isoforms of CAS have been named due to alternative splicing. Isoform 1 is the full length, 971 amino acid protein. Isoform 2 contains an alternative sequence for amino acids 190-195 and is missing amino acids 196-971. Isoform 3 contains an alternative sequence for amino acids 943-945 and is missing amino acids 946-971.