PRODUCT CODE: ET1611-99

Recombinant Cdk8 Monoclonal Antibody (ET1611-99)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Cdk8 on NIH/3T3 cells lysates using anti-Cdk8 antibody at 1/1,000 dilution.
  • Western blot analysis of Cdk8 on NIH/3T3 cells lysates using anti-Cdk8 antibody at 1/1,000 dilution.
Western blot analysis of Cdk8 on NIH/3T3 cells lysates using anti-Cdk8 antibody at 1/1,000 dilution.

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cdk8 Monoclonal Antibody (ET1611-99)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

NIH/3T3.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN2030

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

53 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

Cyclin-dependent kinase 8

GENE NAME

CDK8

SYNONYMS

CDK8

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.