Positive control:
Lane 1: Hela cell lysate
Lane 2: MCF-7 cell lysate
Lane 2: K562 cell lysate
Applications
-
WB
-
ICC
-
IF
-
IP
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Cdk4 Monoclonal Antibody (ET1612-23)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Hela cell lysate, MCF-7 cell lysate, K562 cell lysate, Hela, AGS, NIH/3T3.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SD205-1
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
34 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC/IF
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
Cdk4
SYNONYMS
Cdk 4 antibody; cdk4 antibody; CDK4 protein antibody; CDK4_HUMAN antibody; Cell division kinase 4 antibody; Cell division protein kinase 4 antibody; CMM 3 antibody; CMM3 antibody; Crk3 antibody; Cyclin dependent kinase 4 antibody; Cyclin-dependent kinase 4 antibody; Melanoma cutaneous malignant 3 antibody; MGC14458 antibody; p34 cdk4 antibody; PSK J3 antibody; PSK-J3 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Membrane.
FUNCTION
Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression. Another family of proteins, Cdk inhibitors, also plays a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1-PCTAIRE-3, PITALRE and PITSLRE. Cdk4, in complex with D-type cyclins, is thought to regulate cell growth during the G1 phase of the cell cycle. This association with a D-type cyclin upregulates Cdk4 activity, whereas binding to the Cdk inhibitor p16 downregulates Cdk4 activity. Activation of the Cdk4-cyclin complexes requires phosphorylation on a single threonyl residue of Cdk4, catalyzed by a Cdk-activating protein (CAK).