PRODUCT CODE: ET1701-14

Recombinant Cdc37 Monoclonal Antibody (ET1701-14)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Cdc37 on different lysates using anti-Cdc37 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Jurkat <br />
Lane 2: A431 <br />
Lane 3: K562
  • Western blot analysis of Cdc37 on different lysates using anti-Cdc37 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Jurkat <br />
Lane 2: A431 <br />
Lane 3: K562
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cdc37 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Cdc37 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Cdc37 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of Cdc37 on different lysates using anti-Cdc37 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: A431
Lane 3: K562

Applications

  • WB

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cdc37 Monoclonal Antibody (ET1701-14)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, A431, K562, mouse placenta tissue, mouse liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ087-06

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

45 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • FC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Hsp90 co-chaperone Cdc37

GENE NAME

CDC37

SYNONYMS

CDC37

SEQUENCE SIMILARITIES

Belongs to the CDC37 family.

POST-TRANSLATIONAL MODIFICATION

Constitutively sumoylated by UBE2I.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity. Inhibits HSP90AA1 ATPase activity.