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PRODUCT CODE: ET1701-10

Recombinant Cdc34 Monoclonal Antibody (ET1701-10)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Cdc34 on different lysates using anti-Cdc34 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: K562 <br /> Lane 2: Jurkat
  • Western blot analysis of Cdc34 on different lysates using anti-Cdc34 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: K562 <br /> Lane 2: Jurkat
  • ICC staining Cdc34 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Cdc34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Cdc34 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of Jurkat cells with Cdc34 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Cdc34 on different lysates using anti-Cdc34 antibody at 1/1,000 dilution.
Positive control:
Lane 1: K562
Lane 2: Jurkat

Applications

  • WB

  • ICC

  • IF

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cdc34 Monoclonal Antibody (ET1701-10)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A549, N2A, Hela, K562, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ086-07

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

34 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • FC

  • 1:50-1:100

  • ICC/IF

  • 1:100-1:500

TARGET

UNIPROT #

P49427

PROTEIN NAME

Cdc34

SYNONYMS

Cdc 34 antibody; Cdc34 antibody; Cell division cycle 34 antibody; Cell division cycle 34 homolog (S. cerevisiae) antibody; Cell division cycle 34 homolog antibody; E2 CDC34 antibody; UB2R1_HUMAN antibody; UBC 3 antibody; UBC3 antibody; UBCH3 antibody; UBE2 R1 antibody; UBE2R1 antibody; Ubiquitin carrier protein antibody; Ubiquitin conjugating enzyme Cdc34 antibody; Ubiquitin conjugating enzyme E2 32 kDa complementing antibody; Ubiquitin protein ligase antibody; Ubiquitin protein ligase R1 antibody; Ubiquitin-conjugating enzyme E2 R1 antibody; Ubiquitin-conjugating enzyme E2-32 kDa complementing antibody; Ubiquitin-conjugating enzyme E2-CDC34 antibody; Ubiquitin-protein ligase R1 antibody

SEQUENCE SIMILARITIES

Belongs to the ubiquitin-conjugating enzyme family.

TISSUE SPECIFICITY

Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription.

POST-TRANSLATIONAL MODIFICATION

Autoubiquitinated. Autoubiquitination is promoted by the human herpes virus 1 protein ICP0 and leads to degradation by the Ubiquitin-proteasomal pathway.; Phosphorylated by CK2. Phosphorylation of the C-terminal tail by CK2 controles the nuclear localization.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.