Positive control:
Lane 1: K562
Lane 2: Jurkat
Applications
-
WB
-
ICC
-
IF
-
IP
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Cdc34 Monoclonal Antibody (ET1701-10)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
A549, N2A, Hela, K562, Jurkat.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JJ086-07
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
34 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
-
FC
-
1:50-1:100
-
ICC/IF
-
1:100-1:500
TARGET
UNIPROT #
PROTEIN NAME
Cdc34
SYNONYMS
Cdc 34 antibody; Cdc34 antibody; Cell division cycle 34 antibody; Cell division cycle 34 homolog (S. cerevisiae) antibody; Cell division cycle 34 homolog antibody; E2 CDC34 antibody; UB2R1_HUMAN antibody; UBC 3 antibody; UBC3 antibody; UBCH3 antibody; UBE2 R1 antibody; UBE2R1 antibody; Ubiquitin carrier protein antibody; Ubiquitin conjugating enzyme Cdc34 antibody; Ubiquitin conjugating enzyme E2 32 kDa complementing antibody; Ubiquitin protein ligase antibody; Ubiquitin protein ligase R1 antibody; Ubiquitin-conjugating enzyme E2 R1 antibody; Ubiquitin-conjugating enzyme E2-32 kDa complementing antibody; Ubiquitin-conjugating enzyme E2-CDC34 antibody; Ubiquitin-protein ligase R1 antibody
SEQUENCE SIMILARITIES
Belongs to the ubiquitin-conjugating enzyme family.
TISSUE SPECIFICITY
Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription.
POST-TRANSLATIONAL MODIFICATION
Autoubiquitinated. Autoubiquitination is promoted by the human herpes virus 1 protein ICP0 and leads to degradation by the Ubiquitin-proteasomal pathway.; Phosphorylated by CK2. Phosphorylation of the C-terminal tail by CK2 controles the nuclear localization.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.