Positive control:
Lane 1: Hela
Lane 2: K562
Applications
-
WB
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Cdc27 Monoclonal Antibody (ET1612-94)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
K562, Hela.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SD85-02
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
92 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
TARGET
UNIPROT #
PROTEIN NAME
Cdc27
SYNONYMS
ANAPC3 antibody; Anaphase Promoting Complex 3 antibody; Anaphase promoting complex protein 3 antibody; Anaphase Promoting Complex Subunit 3 antibody; Anaphase-promoting complex subunit 3 antibody; APC 3 antibody; APC3 antibody; Cdc 27 antibody; Cdc27 antibody; CDC27 homolog antibody; CDC27_HUMAN antibody; CDC27Hs antibody; Cell Division Cycle 27 antibody; Cell division cycle protein 27 homolog antibody; D0S1430E antibody; D17S978E antibody; H NUC antibody; H-NUC antibody; HNUC antibody; Nuc2 homolog antibody
SEQUENCE SIMILARITIES
Belongs to the APC3/CDC27 family.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
n the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.