Recombinant Cdc27 Monoclonal Antibody (ET1612-94)

Western blot analysis of Cdc27 on different lysates using anti-Cdc27 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: K562

Western blot analysis of Cdc27 on different lysates using anti-Cdc27 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: K562

Applications

REACTIVITY

Human
Mouse
Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cdc27 Monoclonal Antibody (ET1612-94)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD85-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

92 kDa

Isotype

IgG

APPLICATION DILUTION

WB
1:1,000-1:5,000

TARGET

UNIPROT #

P30260

PROTEIN NAME

Cell division cycle protein 27 homolog

GENE NAME

CDC27

SYNONYMS

APC3, CDC27Hs, CDC27

SEQUENCE SIMILARITIES

Belongs to the APC3/CDC27 family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.