PRODUCT CODE: ET1701-68

Recombinant Cdc25B Monoclonal Antibody (ET1701-68)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

Western blot analysis of Cdc25B on THP-1 cells lysates using anti-Cdc25B antibody at 1/1,000 dilution.
  • Western blot analysis of Cdc25B on THP-1 cells lysates using anti-Cdc25B antibody at 1/1,000 dilution.
Western blot analysis of Cdc25B on THP-1 cells lysates using anti-Cdc25B antibody at 1/1,000 dilution.

Applications

  • WB

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cdc25B Monoclonal Antibody (ET1701-68)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

THP-1.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ091-2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

65 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

M-phase inducer phosphatase 2

GENE NAME

CDC25B

SYNONYMS

CDC25B

SEQUENCE SIMILARITIES

Belongs to the MPI phosphatase family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.

SUBCELLULAR LOCATION

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.

FUNCTION

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.