PRODUCT CODE: ET1701-68

Recombinant Cdc25B Monoclonal Antibody (ET1701-68)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

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Western blot analysis of Cdc25B on THP-1 cells lysates using anti-Cdc25B antibody at 1/1,000 dilution.
  • Western blot analysis of Cdc25B on THP-1 cells lysates using anti-Cdc25B antibody at 1/1,000 dilution.
Western blot analysis of Cdc25B on THP-1 cells lysates using anti-Cdc25B antibody at 1/1,000 dilution.

Applications

  • WB

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cdc25B Monoclonal Antibody (ET1701-68)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

THP-1.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ091-2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

65 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

Cdc25B

SYNONYMS

AI604853 antibody; Cdc 25B antibody; Cdc25b antibody; CDC25HU2 antibody; Cdc25m2 antibody; Cell division cycle 25 homolog B antibody; Cell division cycle 25B antibody; Cell division cycle 25B isoform 1 antibody; Cell division cycle 25B isoform 2 antibody; Cell division cycle 25B isoform 3 antibody; Cell division cycle 25B isoform 4 antibody; Cell division cycle 25B isoform 5 antibody; Dual specificity phosphatase Cdc25B antibody; EC 3.1.3.48 antibody; M phase inducer phosphatase 2 antibody; M-phase inducer phosphatase 2 antibody; MPIP2_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the MPI phosphatase family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

The Cdc2/cyclin B enzyme, involved in regulation of mitosis in eukaryotic cells, is subject to multiple levels of control. Among these, the regulation of the catalytic subunit by Tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B complex, while Tyrosine dephosphorylation, which occurs at the onset of mitosis, directly activates the pre-MPH complex. The Cdc25 gene serves as a rate-limiting mitotic activator, apparently due to its action as the Cdc2 Tyrosine phosphatase. In the absence of Cdc25, Cdc2 accumulates in a Tyrosine phosphorylated state. In addition, Cdc25 proteins from a variety of species have been shown to share a low degree of sequence similarity with other Tyrosine phosphatases. The Cdc25 gene family consists of at least three members that share approximately 40% identity in their most conserved carboxy-terminal sequences.