PRODUCT CODE: ET1612-84

Recombinant CDC23 Monoclonal Antibody (ET1612-84)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Cdc23 on Hela cells lysates using anti-Cdc23 antibody at 1/1,000 dilution.
  • Western blot analysis of Cdc23 on Hela cells lysates using anti-Cdc23 antibody at 1/1,000 dilution.
  • ICC staining Cdc23 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Cdc23 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of Cdc23 on Hela cells lysates using anti-Cdc23 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant CDC23 Monoclonal Antibody (ET1612-84)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD08-60

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

69 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CDC23

SYNONYMS

ANAPC8 antibody; Anaphase promoting complex subunit 8 antibody; Anaphase-promoting complex subunit 8 antibody; Apc 8 antibody; APC8 antibody; Cdc 23 antibody; CDC23 antibody; CDC23_HUMAN antibody; cell division cycle 23 antibody; Cell division cycle 23 homolog antibody; Cell division cycle protein 23 antibody; Cell division cycle protein 23 homolog antibody; Cut23 antibody; Cyclosome subunit 8 antibody

SEQUENCE SIMILARITIES

Belongs to the APC8/CDC23 family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis.

SUBCELLULAR LOCATION

Cytosol.

FUNCTION

Cell cycle progression is controlled at a point late in G1 designated Start. Passage through Start requires the activity of the cyclin-dependent protein kinase Cdc28. Transition from G1 to S phase requires the association of Cdc28 with members of the G1 cyclin family. Exit from mitosis and initiation of the next cell cycle requires a complex of proteins designated the anaphase-promoting complex (APC). This complex consists of two proteins, Cdc16 and Cdc27 (also referred to as Snb1), which are involved in limiting DNA replication to once per cell cycle. Cdc23, another component of the APC, is required for both entering and exiting anaphase, and is important for the proper separation of sister chromatids. The APC is thought to be stabilized by Cdc26 (also known as Scd26). In addition to these APC proteins, Cdc5 is also required for completion of mitosis. In contrast, Cdc20 acts as a DNA-damage induced checkpoint, preventing mitosis when DNA damage has occurred.