PRODUCT CODE: ET1612-84

Recombinant CDC23 Monoclonal Antibody (ET1612-84)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Cdc23 on Hela cells lysates using anti-Cdc23 antibody at 1/1,000 dilution.
  • Western blot analysis of Cdc23 on Hela cells lysates using anti-Cdc23 antibody at 1/1,000 dilution.
  • ICC staining Cdc23 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Cdc23 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of Cdc23 on Hela cells lysates using anti-Cdc23 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant CDC23 Monoclonal Antibody (ET1612-84)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD08-60

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

69 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Cell division cycle protein 23 homolog

GENE NAME

CDC23

SYNONYMS

APC8

SEQUENCE SIMILARITIES

Belongs to the APC8/CDC23 family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis.

FUNCTION

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.