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PRODUCT CODE: ET1611-53

Recombinant CD68 Monoclonal Antibody (ET1611-53)

  • Recombinant

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of CD68 on SH-SY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of CD68 on SH-SY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD68 on SH-SY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant CD68 Monoclonal Antibody (ET1611-53)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SH-SY-5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN07-27

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

37 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

TARGET

UNIPROT #

P34810

PROTEIN NAME

CD68

SYNONYMS

CD 68 antibody; CD68 antibody; CD68 antigen antibody; CD68 molecule antibody; CD68_HUMAN antibody; DKFZp686M18236 antibody; gp11 antibody; Gp110 antibody; LAMP4 antibody; Macrophage antigen CD68 (microsialin) antibody; MACROPHAGE ANTIGEN CD68 antibody; Macrosialin antibody; SCARD1 antibody; Scavenger receptor class D member 1 antibody

SEQUENCE SIMILARITIES

Belongs to the LAMP family.

TISSUE SPECIFICITY

Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.

POST-TRANSLATIONAL MODIFICATION

N- and O-glycosylated.

SUBCELLULAR LOCATION

Cell membrane, Endosome membrane, Lysosome membrane.

FUNCTION

CD68, also called Scavenger Receptor D1 (SR-D1) or macrosialin in mouse, is a 110 kDa type I transmembrane glycoprotein that belongs to the LAMP family of molecules. It contains a 300 amino acid (aa) extracellular region that is rich in threonine and serine, a likely attachment site for multiple carbohydrates. Human CD68 shares 74% aa sequence identity to mouse CD68 in the extracellular region. CD68 is found on monocytes and macrophages and serves as a scavenger receptor for oxidized LDL.