PRODUCT CODE: ET1607-2

Recombinant CD63 Monoclonal Antibody (ET1607-2)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of CD63 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-2, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control:<br />
 Lane 1: HL-60 cell lysate<br />
 Lane 2: THP-1 cell lysate<br />
 Lane 3: NIH/3T3 cell lysate
  • Western blot analysis of CD63 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-2, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control:<br />
 Lane 1: HL-60 cell lysate<br />
 Lane 2: THP-1 cell lysate<br />
 Lane 3: NIH/3T3 cell lysate
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-CD63 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-2, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD63 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-2, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of CD63 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-2, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HL-60 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: NIH/3T3 cell lysate

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant CD63 Monoclonal Antibody (ET1607-2)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HL-60 cell lysate, THP-1 cell lysate, NIH/3T3 cell lysate, human lung carcinoma tissue, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SY21-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

23 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CD63

SYNONYMS

Lysosomal associated membrane protein 3 antibody; CD 63 antibody; CD63 antibody; CD63 antigen (melanoma 1 antigen) antibody; CD63 antigen antibody; CD63 antigen melanoma 1 antigen antibody; CD63 molecule antibody; CD63_HUMAN antibody; gp55 antibody; Granulophysin antibody; LAMP 3 antibody; LAMP-3 antibody; LAMP3 antibody; LIMP antibody; Lysosomal-associated membrane protein 3 antibody; Lysosome associated membrane glycoprotein 3 antibody; Mast cell antigen AD1 antibody; ME491 antibody; Melanoma 1 antigen antibody; Melanoma associated antigen ME491 antibody; Melanoma associated antigen MLA1 antibody; Melanoma-associated antigen ME491 antibody; MGC72893 antibody; MLA 1 antibody; MLA1 antibody; NGA antibody; Ocular melanoma associated antigen antibody; Ocular melanoma-associated antigen antibody; OMA81H antibody; PTLGP40 antibody; Tetraspanin 30 antibody; Tetraspanin-30 antibody; Tspan 30 antibody; Tspan-30 antibody; TSPAN30 antibody

SEQUENCE SIMILARITIES

Belongs to the tetraspanin (TM4SF) family.

TISSUE SPECIFICITY

Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.

POST-TRANSLATIONAL MODIFICATION

Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro).

SUBCELLULAR LOCATION

Cell membrane, Lysosome membrane, Endosome, Melanosome, Secreted.

FUNCTION

The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 (also known as LAMP-3, Melanoma-associated antigen ME491, TSPAN30, MLA1 and OMA81H) is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome.

CITATIONS

  • Zhang, Yuefeng et al.

    Pancreatic cancer-derived exosomes suppress the production of GIP and GLP-1 from STC-1���cells in vitro by down-regulating the PCSK1/3. | Cancer Letters [2018]

  • He, Xiaoqin et al.

    Exosomes derived from liver cancer cells reprogram biological behaviors of LO2 cells by transferring Linc-ROR. | Gene [2019]