PRODUCT CODE: ET1703-49

Recombinant CD62P Monoclonal Antibody (ET1703-49)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of CD62P on different cells lysates using anti-CD62P antibody at 1/500 dilution.<br />
Positive control: <br />
Line1: HUVEC <br />
Line2: Human placenta
  • Western blot analysis of CD62P on different cells lysates using anti-CD62P antibody at 1/500 dilution.<br />
Positive control: <br />
Line1: HUVEC <br />
Line2: Human placenta
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD62P antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD62P antibody. Counter stained with hematoxylin.
Western blot analysis of CD62P on different cells lysates using anti-CD62P antibody at 1/500 dilution.
Positive control:
Line1: HUVEC
Line2: Human placenta

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant CD62P Monoclonal Antibody (ET1703-49)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HUVEC, human placenta, human tonsil tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-95

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

100 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

P-selectin

GENE NAME

SELP

SYNONYMS

GMP-140, LECAM3, PADGEM, SELP

SEQUENCE SIMILARITIES

Belongs to the selectin/LECAM family.

TISSUE SPECIFICITY

Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

SUBCELLULAR LOCATION

Cell membrane; Single-pass type I membrane protein.

FUNCTION

Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG.