PRODUCT CODE: ET1703-28

Recombinant CD59 Monoclonal Antibody (ET1703-28)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • FC

  • IP

REACTIVITY

  • Human

Western blot analysis of CD59 on different cells lysates using anti-CD59 antibody at 1/500 dilution.<br />
Positive control:<br />
Lane 1: Human placenta<br />
Lane 2: HUVEC<br />
Lane 3: K562
  • Western blot analysis of CD59 on different cells lysates using anti-CD59 antibody at 1/500 dilution.<br />
Positive control:<br />
Lane 1: Human placenta<br />
Lane 2: HUVEC<br />
Lane 3: K562
  • ICC staining CD59 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD59 in JAR cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD59 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of NIH-3T3 cells with CD59 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of CD59 on different cells lysates using anti-CD59 antibody at 1/500 dilution.
Positive control:
Lane 1: Human placenta
Lane 2: HUVEC
Lane 3: K562

Applications

  • WB

  • ICC

  • IF

  • FC

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant CD59 Monoclonal Antibody (ET1703-28)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

NIH-3T3, HUVEC, JAR, K562, Human placenta.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-71

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

14/20 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:100-1:500

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

CD59 glycoprotein

GENE NAME

CD59

SYNONYMS

HRF-20, HRF20, MAC-IP, MACIF, MIRL, CD59

POST-TRANSLATIONAL MODIFICATION

N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine. Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.; Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes.

SUBCELLULAR LOCATION

Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Note=Soluble form found in a number of tissues.

FUNCTION

Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.; The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.