PRODUCT CODE: ET1703-64

Recombinant CD40L Monoclonal Antibody (ET1703-64)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of CD40L on different cells lysates using anti-CD40L antibody at 1/500 dilution.<br />
Positive control:<br />
Lane 1: Hela<br />
Lane 2: Mouse liver
  • Western blot analysis of CD40L on different cells lysates using anti-CD40L antibody at 1/500 dilution.<br />
Positive control:<br />
Lane 1: Hela<br />
Lane 2: Mouse liver
  • ICC staining CD40L in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD40L in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD40L in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti- CD40L antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of THP-1 cells with CD40L antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of CD40L on different cells lysates using anti-CD40L antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: Mouse liver

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant CD40L Monoclonal Antibody (ET1703-64)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, MCF-7, NIH-3T3, THP-1, mouse liver cells lysates, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-34

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

36 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CD40 ligand

GENE NAME

CD40LG

SYNONYMS

CD40-L, TRAP, CD40LG

SEQUENCE SIMILARITIES

Belongs to the tumor necrosis factor family.

TISSUE SPECIFICITY

Specifically expressed on activated CD4+ T-lymphocytes.

POST-TRANSLATIONAL MODIFICATION

The soluble form derives from the membrane form by proteolytic processing.; N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.; Not O-glycosylated.

SUBCELLULAR LOCATION

Cell membrane; Single-pass type II membrane protein. Cell surface.; [CD40 ligand, soluble form]: Secreted. Note=Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and a matrix metalloproteinases (MMP) inhibitor-sensitive pathway.

FUNCTION

Cytokine that acts as a ligand to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B. Induces the activation of kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity).; [CD40 ligand, soluble form]: Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.