PRODUCT CODE: ET7107-55

Recombinant CD3G Monoclonal Antibody (ET7107-55)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

Western blot analysis of CD3G on mouse thymus tissue lysate using anti-CD3G antibody at 1/500 dilution.
  • Western blot analysis of CD3G on mouse thymus tissue lysate using anti-CD3G antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD3G antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD3G antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with CD3G antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of CD3G on mouse thymus tissue lysate using anti-CD3G antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant CD3G Monoclonal Antibody (ET7107-55)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat cell lysates, mouse thymus tissue lysates, human tonsil tissue, human spleen tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB38-29

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

20 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

CD3G

SYNONYMS

CD3 gamma antibody; CD3-GAMMA antibody; CD3g antibody; CD3g antigen gamma polypeptide (TiT3 complex) antibody; CD3g antigen gamma polypeptide antibody; CD3g molecule gamma (CD3 TCR complex) antibody; CD3g molecule gamma antibody; CD3G_HUMAN antibody; FLJ17620 antibody; FLJ17664 antibody; FLJ79544 antibody; FLJ94613 antibody; MGC138597 antibody; T cell antigen receptor complex gamma subunit of T3 antibody; T-cell receptor T3 gamma chain antibody; T-cell surface glycoprotein CD3 gamma chain antibody; T3G antibody

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8. Phosphorylated also by PKC; leading to the TCR complex down-regulation.; Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of gamma and epsilon chains (γε), a heterodimer of delta and epsilon chains (δε) and a homodimer of two zeta chains (ζζ) or a heterodimer of zeta and eta chains (ζη). The ζ and η chains are encoded by the same gene but differ in their carboxyl-terminal ends due to an alternative splicing event. The γ, ε and δ chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM).