Positive control:
Lane 1: TF-1 cell lysate
Lane 2: Mouse lung tissue lysate
Applications
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WB
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ICC
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IF
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IHC-P
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IP
REACTIVITY
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Human
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Mouse
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Rat
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Dog
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant CD34 Monoclonal Antibody (ET1606-11)
Immunogen
Synthetic peptide within c-terminal of human cd34.
Host
Rabbit
Positive Control
TF-1 cell lysate, mouse lung tissue lysate, A549, HUVEC, SH-SY5Y, human tonsil tissue, human kidney tissue, mouse kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SI16-01
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
100/120 kDa (Observed band size)
Isotype
IgG
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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ICC/IF
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1:50-1:200
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IHC-P
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1:50-1:200
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IP
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assay-dependent
TARGET
UNIPROT #
PROTEIN NAME
CD34
SYNONYMS
CD34 antibody; CD34 antigen antibody; CD34 molecule antibody; CD34_HUMAN antibody; Cluster designation 34 antibody; Hematopoietic progenitor cell antigen CD34 antibody; HPCA1 antibody; Mucosialin antibody; OTTHUMP00000034733 antibody; OTTHUMP00000034734 antibody
SEQUENCE SIMILARITIES
Belongs to the CD34 family.
TISSUE SPECIFICITY
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
DEVELOPMENTAL STAGE
On early hematopoietic progenitor cells.
POST-TRANSLATIONAL MODIFICATION
Highly glycosylated.; Phosphorylated on serine residues by PKC.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed on the surface of lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult nervous tissue. CD34 antigen expression is highest in the most primitive stem cells and is gradually lost as lineage committed progenitors differentiate. The CD34 antigen is also present on capillary endothelial cells and on bone marrow stromal cells. The CD34 cytoplasmic domain has an intracellular domain that contains consensus sites for activated protein kinase C (PKC) phosphorylation as well as serine, threonine and tyrosine phosphorylation consensus sites.
CITATIONS
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Wang, Di et al.
Effect of cold stress on ovarian & uterine microcirculation in rats and the role of endothelin system. | Reproductive Biology and Endocrinology : Rb&E [2020]
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IHC
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rat
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Citation
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