Lane 1: Daudi cell lysate
Lane 2: 293 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: rat brain tissue lysate
Recombinant Rabbit monoclonal primary
Recombinant CD19 Monoclonal Antibody (ET1702-74)
Daudi cell lysate, 293 cell lysate, Jurkat cell lysate, rat brain tissue lysate, Jurkat.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
antibody; deficiency due to defect in CD19, included antibody; AW495831 antibody; B lymphocyte antigen CD19 antibody; B lymphocyte surface antigen B4 antibody; B-lymphocyte antigen CD19 antibody; B-lymphocyte surface antigen B4 antibody; B4 antibody; CD19 antibody; CD19 antigen antibody; CD19 molecule antibody; Cd19 protein antibody; CD19_HUMAN antibody; CVID3 antibody; Differentiation antigen CD19 antibody; Leu 12 antibody; Leu-12 antibody; Leu12 antibody; MGC109570 antibody; MGC12802 antibody; T-cell surface antigen Leu-12 antibody
Detected on marginal zone and germinal center B cells in lymph nodes. Detected on blood B cells (at protein level).
Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. In contrast, phosphorylation of Tyr-500 appears more slowly and is more transient, returning rapidly to basal levels (By similarity).
Membrane, Membrane raft.
CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. CD19 is selectively expressed on the cell surface of B-lymphocytes, where it activates intracellular signaling cascades involving both Ras and phosphatidylinositol 3-kinase pathways. Activation of CD19 results in cross-linking of the membrane protein immunoglobulin chains and the subsequent association with Src family protein tyrosine kinases (PTK). Expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells. CD19 forms functional complexes with B-lymphocyte surface proteins, including integrin b1, CD21 and CD81, which are involved in regulating B-cell development.