PRODUCT CODE: ET1702-74

Recombinant CD19 Monoclonal Antibody (ET1702-74)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of CD19 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: Daudi cell lysate<br />
 Lane 2: 293 cell lysate<br />
 Lane 3: Jurkat cell lysate<br />
 Lane 4: rat brain tissue lysate
  • Western blot analysis of CD19 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: Daudi cell lysate<br />
 Lane 2: 293 cell lysate<br />
 Lane 3: Jurkat cell lysate<br />
 Lane 4: rat brain tissue lysate
  • Flow cytometric analysis of CD19 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-74, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of CD19 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Daudi cell lysate
Lane 2: 293 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: rat brain tissue lysate

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant CD19 Monoclonal Antibody (ET1702-74)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Daudi cell lysate, 293 cell lysate, Jurkat cell lysate, rat brain tissue lysate, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF099-9

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

75-100 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • FC

  • 1:10-1:100

TARGET

UNIPROT #

PROTEIN NAME

CD19

SYNONYMS

antibody; deficiency due to defect in CD19, included antibody; AW495831 antibody; B lymphocyte antigen CD19 antibody; B lymphocyte surface antigen B4 antibody; B-lymphocyte antigen CD19 antibody; B-lymphocyte surface antigen B4 antibody; B4 antibody; CD19 antibody; CD19 antigen antibody; CD19 molecule antibody; Cd19 protein antibody; CD19_HUMAN antibody; CVID3 antibody; Differentiation antigen CD19 antibody; Leu 12 antibody; Leu-12 antibody; Leu12 antibody; MGC109570 antibody; MGC12802 antibody; T-cell surface antigen Leu-12 antibody

TISSUE SPECIFICITY

Detected on marginal zone and germinal center B cells in lymph nodes. Detected on blood B cells (at protein level).

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. In contrast, phosphorylation of Tyr-500 appears more slowly and is more transient, returning rapidly to basal levels (By similarity).

SUBCELLULAR LOCATION

Membrane, Membrane raft.

FUNCTION

CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. CD19 is selectively expressed on the cell surface of B-lymphocytes, where it activates intracellular signaling cascades involving both Ras and phosphatidylinositol 3-kinase pathways. Activation of CD19 results in cross-linking of the membrane protein immunoglobulin chains and the subsequent association with Src family protein tyrosine kinases (PTK). Expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells. CD19 forms functional complexes with B-lymphocyte surface proteins, including integrin b1, CD21 and CD81, which are involved in regulating B-cell development.