PRODUCT CODE: ET1703-94

Recombinant CD18 Monoclonal Antibody (ET1703-94)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

Western blot analysis of CD18 on Jurkat cells lysates using anti-CD18 antibody at 1/500 dilution.
  • Western blot analysis of CD18 on Jurkat cells lysates using anti-CD18 antibody at 1/500 dilution.
Western blot analysis of CD18 on Jurkat cells lysates using anti-CD18 antibody at 1/500 dilution.

Applications

  • WB

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant CD18 Monoclonal Antibody (ET1703-94)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM71-31

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

95 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Integrin beta-2

GENE NAME

ITGB2

SYNONYMS

ITGB2

SEQUENCE SIMILARITIES

Belongs to the integrin beta chain family.

TISSUE SPECIFICITY

Leukocytes. Expressed in neutrophils (at protein level).

POST-TRANSLATIONAL MODIFICATION

Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.

SUBCELLULAR LOCATION

Cell membrane; Single-pass type I membrane protein; Single-pass type I membrane protein.

FUNCTION

Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL. Integrins ITGAM/ITGB2 and ITGAX/ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX/ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM/ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM/ITGB2 is also a receptor for factor X. Integrin ITGAD/ITGB2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages. In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils.