PRODUCT CODE: ET1611-61

Recombinant CD13 Monoclonal Antibody (ET1611-61)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of CD13 on different lysates using anti-CD13 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: THP-1 <br />
 Lane 2: Human kidney
  • Western blot analysis of CD13 on different lysates using anti-CD13 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: THP-1 <br />
 Lane 2: Human kidney
  • ICC staining CD13 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS
  • ICC staining CD13 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD13 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD13 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD13 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-CD13 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD13 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-CD13 antibody. Counter stained with hematoxylin.
Western blot analysis of CD13 on different lysates using anti-CD13 antibody at 1/1,000 dilution.
Positive control:
Lane 1: THP-1
Lane 2: Human kidney

Applications

  • WB

  • ICC

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant CD13 Monoclonal Antibody (ET1611-61)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse kidney tissue lysate, THP-1 cell lysate, human kidney tissue lysate, Hela, HepG2, PANC-1, human tonsil tissue, human liver tissue, human breast tissue, human kidney tissue, mouse pancreas tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN71-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

150 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

CD13

SYNONYMS

Alanyl (membrane) aminopeptidase antibody; Alanyl aminopeptidase antibody; Aminopeptidase M antibody; Aminopeptidase N antibody; AMPN_HUMAN antibody; ANPEP antibody; AP M antibody; AP N antibody; AP-M antibody; AP-N antibody; APN antibody; CD 13 antibody; CD13 antibody; CD13 antigen antibody; gp150 antibody; hAPN antibody; LAP 1 antibody; LAP1 antibody; Microsomal aminopeptidase antibody; Myeloid plasma membrane glycoprotein CD13 antibody; p150 antibody; PEPN antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase M1 family.

TISSUE SPECIFICITY

Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.

POST-TRANSLATIONAL MODIFICATION

Sulfated.; N- and O-glycosylated.; May undergo proteolysis and give rise to a soluble form.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

CD13, or aminopeptidase N, is a type II transmembrane glycoprotein that is expressed on most cells of Myeloid origin, including monocytes, basophils, eosinophils, neutrophils and Myeloid leukemias. CD13 is also found on certain epithelial cells, fibroblasts and osteoclasts. CD13 acts as a zinc-binding metalloprotease that plays a role in digestion and may function in the inactivation of some regulatory peptides such as enkephalins. CD13 may play a role in the invasion of cancer cells by enhancing their invasive capacity and metastatic behavior. The activity of CD13 can be inactivated using specific inhibitors that evoke apoptosis of CD13-positive cancer cells. Basic fibroblast growth factor (bFGF) expression upregulates CD13 expression in human melanoma cells by activating both the Myeloid and the epithelial CD13 promoter.