PRODUCT CODE: ET1611-61

Recombinant CD13 Monoclonal Antibody (ET1611-61)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of CD13 on different lysates using anti-CD13 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: THP-1 <br />
Lane 2: Human kidney
  • Western blot analysis of CD13 on different lysates using anti-CD13 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: THP-1 <br />
Lane 2: Human kidney
  • ICC staining CD13 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS
  • ICC staining CD13 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD13 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD13 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD13 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-CD13 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD13 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-CD13 antibody. Counter stained with hematoxylin.
Western blot analysis of CD13 on different lysates using anti-CD13 antibody at 1/1,000 dilution.
Positive control:
Lane 1: THP-1
Lane 2: Human kidney

Applications

  • WB

  • ICC

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant CD13 Monoclonal Antibody (ET1611-61)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PANC-1, THP-1, Hela, HepG2, human kidney tissue, mouse pancreas tissue, human tonsil tissue, human liver tissue, human breast tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN71-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

150 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

Aminopeptidase N

GENE NAME

ANPEP

SYNONYMS

AP-N, hAPN, AP-M, ANPEP

SEQUENCE SIMILARITIES

Belongs to the peptidase M1 family.

TISSUE SPECIFICITY

Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.

POST-TRANSLATIONAL MODIFICATION

Sulfated.; N- and O-glycosylated.; May undergo proteolysis and give rise to a soluble form.

SUBCELLULAR LOCATION

Cell membrane; Single-pass type II membrane protein. Note=Also found as a soluble form.

FUNCTION

Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity (By similarity).; (Microbial infection) Acts as a receptor for human coronavirus 229E/HCoV-229E. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein.; (Microbial infection) Mediates as well Human cytomegalovirus (HCMV) infection.