PRODUCT CODE: ET1607-15

Recombinant Caveolin-2 Monoclonal Antibody (ET1607-15)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

Western blot analysis of caveolin-2 on HUVEC cell lysates using anti-caveolin-2 antibody at 1/1,000 dilution.
  • Western blot analysis of caveolin-2 on HUVEC cell lysates using anti-caveolin-2 antibody at 1/1,000 dilution.
  • ICC staining caveolin-2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human cervix uteri tissue using anti-caveolin-2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-caveolin-2 antibody. Counter stained with hematoxylin. <br />
Western blot analysis of caveolin-2 on HUVEC cell lysates using anti-caveolin-2 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Caveolin-2 Monoclonal Antibody (ET1607-15)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HUVEC, Hela, human cervix uteri tissue, human uterus tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SY22-05

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

20 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Caveolin-2

GENE NAME

CAV2

SYNONYMS

CAV2

SEQUENCE SIMILARITIES

Belongs to the caveolin family.

TISSUE SPECIFICITY

Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin (By similarity).

SUBCELLULAR LOCATION

Nucleus. Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Note=Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.

FUNCTION

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression (By similarity).