PRODUCT CODE: ET1704-03

Recombinant Cathepsin B Monoclonal Antibody (ET1704-03)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of Cathepsin B on different cells lysates using anti-Cathepsin B antibody at 1/500 dilution.<br />
Positive control: <br />
 Lane 1: HepG2 <br />
 Lane 2: Hela <br />
 Lane 3:A549
  • Western blot analysis of Cathepsin B on different cells lysates using anti-Cathepsin B antibody at 1/500 dilution.<br />
Positive control: <br />
 Lane 1: HepG2 <br />
 Lane 2: Hela <br />
 Lane 3:A549
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Cathepsin B antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cathepsin B antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Cathepsin B antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cathepsin B antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Cathepsin B antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Cathepsin B antibody. Counter stained with hematoxylin.
Western blot analysis of Cathepsin B on different cells lysates using anti-Cathepsin B antibody at 1/500 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Hela
Lane 3:A549

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cathepsin B Monoclonal Antibody (ET1704-03)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2, A549, Hela, human liver tissue, human liver cancer tissue, human pancreas tissue, mouse pancreas tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA11-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Cathepsin B

GENE NAME

CTSB

SYNONYMS

APPS, CTSB

SEQUENCE SIMILARITIES

Belongs to the peptidase C1 family.

TISSUE SPECIFICITY

Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum.

SUBCELLULAR LOCATION

Lysosome. Melanosome. Secreted, extracellular space.

FUNCTION

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity). Has also been implicated in tumor invasion and metastasis.