PRODUCT CODE: ET1610-24

Recombinant Caspase-6 Monoclonal Antibody (ET1610-24)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Caspase-6 on different cell lysates using anti-Caspase-6 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Mouse colon <br />
 Lane 2: MCF-7
  • Western blot analysis of Caspase-6 on different cell lysates using anti-Caspase-6 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Mouse colon <br />
 Lane 2: MCF-7
  • Western blot analysis of Caspase-6 on different cell lysates using anti-Caspase-6 antibody at 1/500 dilution. <br />
 Positive control: <br />
 Lane 1: 3T3 <br />
 Lane 2:mouse kidney
  • ICC staining Caspase-6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Caspase-6 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caspase-6 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Caspase-6 antibody. Counter stained with hematoxylin.
Western blot analysis of Caspase-6 on different cell lysates using anti-Caspase-6 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse colon
Lane 2: MCF-7

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Caspase-6 Monoclonal Antibody (ET1610-24)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SW480, MCF-7, human breast carcinoma tissue, human kidney tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC56-09

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

11/33 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Caspase-6

SYNONYMS

Apoptotic protease Mch-2 antibody; Apoptotic protease MCH2 antibody; CASP-6 antibody; CASP6 antibody; CASP6_HUMAN antibody; Caspase 6 antibody; Caspase 6 apoptosis related cysteine protease antibody; Caspase 6, apoptosis related cysteine peptidase antibody; Caspase-6 subunit p11 antibody; Mch2 antibody; OTTHUMP00000162742 antibody; OTTHUMP00000162743 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C14A family.

POST-TRANSLATIONAL MODIFICATION

Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is comprised of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6, caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9 and caspase-10. Ced-3/ caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Poly(ADP-ribose)polymerase plays an integral role in surveying for DNA mutations and double strand breaks. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the nuclear protein PARP into an apoptotic fragment. caspase-6, but not caspase-3, has been shown to cleave the nuclear lamins, which are critical to maintaining the integrity of the nuclear envelope and cellular morphology. Caspase-10 has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli.