PRODUCT CODE: ET1701-53

Recombinant Caspase-10 Monoclonal Antibody (ET1701-53)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

Western blot analysis of Caspase-10 on K562 cells lysates using anti-Caspase-10 antibody at 1/1,000 dilution.
  • Western blot analysis of Caspase-10 on K562 cells lysates using anti-Caspase-10 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Caspase-10 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caspase-10 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of K562 cells with Caspase-10 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of Caspase-10 on K562 cells lysates using anti-Caspase-10 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Caspase-10 Monoclonal Antibody (ET1701-53)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562, human liver cancer tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ0890

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

59 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Caspase-10

SYNONYMS

ALPS2 antibody; Apoptotic protease Mch-4 antibody; CASP 10 antibody; CASP-10 antibody; CASP10 antibody; CASPA_HUMAN antibody; Caspase 10 apoptosis related cysteine peptidase antibody; Caspase-10 subunit p12 antibody; FADD like ICE2 antibody; Fas associated death domain protein antibody; FAS-associated death domain protein interleukin-1B-converting enzyme 2 antibody; FLICE 2 antibody; FLICE2 antibody; ICE like apoptotic protease 4 antibody; ICE-like apoptotic protease 4 antibody; Interleukin 1B converting enzyme 2 antibody; MCH 4 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C14A family.

TISSUE SPECIFICITY

Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.

POST-TRANSLATIONAL MODIFICATION

Cleavage by granzyme B and autocatalytic activity generate the two active subunits.

SUBCELLULAR LOCATION

Cytosol.

FUNCTION

Caspase-10, also designated Mch4, is recruited to the native TRAIL and CD9 death-inducing signaling complexes (DISCs) by the FADD/Mort1 adaptor protein complex. Caspase-10 requires the assembly of the FADD and DISC complexes for its recruitment and cleavage-induced activation during CD95-induced apoptosis of activated T cells. The N-terminus of caspase-10 contains FADD-like death effector domains further indicating that it associates with FADD to induce apoptosis. Caspase-10 is not required for apoptosis induction and when overexpressed, cannot reverse defects in apoptosis induction caused by caspase-8 deficiency. Granzyme B cleaves procaspase-10 at an IXXD-A processing sequence to produce mature caspase-10. Mutations in the caspase-10 gene in the prodomain, p17 large protease subunit and p12 small protease subunit have been linked to a number of non-Hodgkin lymphomas in humans.