PRODUCT CODE: ET1702-37

Recombinant Cardiac Troponin I Monoclonal Antibody (ET1702-37)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Troponin I on human heart lysates using anti-Troponin I antibody at 1/1,000 dilution.
  • Western blot analysis of Troponin I on human heart lysates using anti-Troponin I antibody at 1/1,000 dilution.
  • ICC staining Troponin I in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Troponin I in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-Troponin I antibody. Counter stained with hematoxylin.
Western blot analysis of Troponin I on human heart lysates using anti-Troponin I antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cardiac Troponin I Monoclonal Antibody (ET1702-37)

Immunogen

Recombinant protein.

Host

Rabbit

Positive Control

NIH/3T3, HepG2, human heart tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF0550

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

24 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Troponin I, cardiac muscle

GENE NAME

TNNI3

SYNONYMS

TNNI3

SEQUENCE SIMILARITIES

Belongs to the troponin I family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T).

FUNCTION

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.