PRODUCT CODE: ET1701-51

Recombinant Carbonic anhydrase 9 Monoclonal Antibody (ET1701-51)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of Carbonic anhydrase 9 on human lung lysates using anti- Carbonic anhydrase 9 antibody at 1/1,000 dilution.
  • Western blot analysis of Carbonic anhydrase 9 on human lung lysates using anti- Carbonic anhydrase 9 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti- Carbonic anhydrase 9 antibody. Counter stained with hematoxylin.
Western blot analysis of Carbonic anhydrase 9 on human lung lysates using anti- Carbonic anhydrase 9 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Carbonic anhydrase 9 Monoclonal Antibody (ET1701-51)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human lung tissue, human liver cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ088-9

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Carbonic anhydrase 9

SYNONYMS

CA-IX antibody; CA9 antibody; CAH9_HUMAN antibody; CAIX antibody; Carbonate dehydratase IX antibody; Carbonic anhydrase 9 antibody; Carbonic anhydrase IX antibody; Carbonic dehydratase antibody; G250 antibody; Membrane antigen MN antibody; MN antibody; P54/58N antibody; pMW1 antibody; RCC associated protein G250 antibody; RCC-associated antigen G250 antibody; Renal cell carcinoma-associated antigen G250 antibody

SEQUENCE SIMILARITIES

Belongs to the alpha-carbonic anhydrase family.

TISSUE SPECIFICITY

Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

POST-TRANSLATIONAL MODIFICATION

Asn-346 bears high-mannose type glycan structures.

SUBCELLULAR LOCATION

Nucleus, Cell membrane, Cell projection.

FUNCTION

Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes including respiration, calcification, acid-base balance and bone resorption, as well as the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA2 gene, which maps to chromosome 8q21, encodes CA II, a cytoplasmic protein that has the highest turnover rate and widest tissue distribution of any known human CA isozyme. The human CA4 gene, which maps to chromosome 17q23, encodes CA IV, a membrane-anchored isozyme that is expressed on the luminal surfaces of pulmonary capillaries and proximal renal tubules. The human CA9, CA12 and CA14 genes, which map to chromosomes 9p13, 15q22 and 1q21, respectively, encode transmembrane proteins that have unique patterns of tissue-specific expression. CA IX is specifically expressed in clear-cell renal carcinomas, whereas CA XII is highly expressed in normal tissues, such as kidney, colon and pancreas. Human CA XIV is also expressed in normal tissues, such as brain, but differs from CA XII in its expression pattern.