PRODUCT CODE: ET1704-37

Recombinant CaMKII delta Monoclonal Antibody (ET1704-37)

  • Zebrafish
  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

Western blot analysis of CaMKII delta on Human skeletal muscle (1) and Rat heart (2) cells lysates using anti-CaMKII delta antibody at 1/500 dilution.
  • Western blot analysis of CaMKII delta on Human skeletal muscle (1) and Rat heart (2) cells lysates using anti-CaMKII delta antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-CaMKII delta antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CaMKII delta antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-CaMKII delta antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CaMKII delta antibody. Counter stained with hematoxylin.
Western blot analysis of CaMKII delta on Human skeletal muscle (1) and Rat heart (2) cells lysates using anti-CaMKII delta antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant CaMKII delta Monoclonal Antibody (ET1704-37)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human skeletal muscle tissue lysate, rat heart tissue lysate, zebrafish tissue, rat brain tissue, mouse heart tissue, mouse skeletal muscle tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA30-07

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Calcium/calmodulin-dependent protein kinase type II subunit delta

GENE NAME

CAMK2D

SYNONYMS

CaM kinase II subunit delta, CaMK-II subunit delta, CAMK2D, CAMKD

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

TISSUE SPECIFICITY

Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylation of Thr-287 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-287 locks the kinase into an activated state.

SUBCELLULAR LOCATION

Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side. Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.

FUNCTION

Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca(2+) homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca(2+) influx into the myocyte, Ca(2+) release from the sarcoplasmic reticulum (SR), SR Ca(2+) uptake and Na(+) and K(+) channel transport. Targets also transcription factors and signaling molecules to regulate heart function. In its activated form, is involved in the pathogenesis of dilated cardiomyopathy and heart failure. Contributes to cardiac decompensation and heart failure by regulating SR Ca(2+) release via direct phosphorylation of RYR2 Ca(2+) channel on 'Ser-2808'. In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program. Is essential for left ventricular remodeling responses to myocardial infarction. In pathological myocardial remodeling acts downstream of the beta adrenergic receptor signaling cascade to regulate key proteins involved in ECC. Regulates Ca(2+) influx to myocytes by binding and phosphorylating the L-type Ca(2+) channel subunit beta-2 CACNB2. In addition to Ca(2+) channels, can target and regulate the cardiac sarcolemmal Na(+) channel Nav1.5/SCN5A and the K+ channel Kv4.3/KCND3, which contribute to arrhythmogenesis in heart failure. Phosphorylates phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2, contributing to the enhancement of SR Ca(2+) uptake that may be important in frequency-dependent acceleration of relaxation (FDAR) and maintenance of contractile function during acidosis. May participate in the modulation of skeletal muscle function in response to exercise, by regulating SR Ca(2+) transport through phosphorylation of PLN/PLB and triadin, a ryanodine receptor-coupling factor.