PRODUCT CODE: ET1703-54

Recombinant CaMKII alpha Monoclonal Antibody (ET1703-54)

  • Recombinant

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of CaMKII alpha on rat brain cells lysates using anti-CaMKII alpha antibody at 1/500 dilution.
  • Western blot analysis of CaMKII alpha on rat brain cells lysates using anti-CaMKII alpha antibody at 1/500 dilution.
  • Flow cytometric analysis of SH-SY5Y cells with CaMKII alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the _x0018_secondary antibody.
Western blot analysis of CaMKII alpha on rat brain cells lysates using anti-CaMKII alpha antibody at 1/500 dilution.

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant CaMKII alpha Monoclonal Antibody (ET1703-54)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SH-SY5Y, rat brain cells.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-07

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

54 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

CaMKII alpha

SYNONYMS

Alpha CaMKII antibody; Calcium calmodulin dependent protein kinase II antibody; Calcium/calmodulin dependent protein kinase II alpha B subunit antibody; Calcium/calmodulin dependent protein kinase type II alpha chain antibody; Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha antibody; Calcium/calmodulin-dependent protein kinase II alpha antibody; Calcium/calmodulin-dependent protein kinase II-alpha antibody; Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody; Calcium/calmodulin-dependent protein kinase type IIA antibody; CaM kinase II alpha chain antibody; CaM kinase II alpha subunit antibody; CaM kinase II subunit alpha antibody; CaMK II alpha subunit antibody; CaMK-II subunit alpha antibody; Camk2a antibody; CAMKA antibody; CaMKII antibody; CaMKIINalpha antibody; EC 2.7.11.17 antibody; KCC2A_HUMAN antibody; KIAA0968 antibody; MGC123320 antibody; MGC139375 antibody; MGC155201 antibody; mKIAA0968 antibody; PK2CDD antibody; PKCCD antibody; R74975 antibody; zgc:112538 antibody; zgc:123320 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylation of Thr-286 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-286 locks the kinase into an activated state.; Palmitoylated. Probably palmitoylated by ZDHHC3 and ZDHHC7.

SUBCELLULAR LOCATION

Cell junction, synapse, presynaptic cell membrane.

FUNCTION

The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177.