PRODUCT CODE: ET7107-39

Recombinant Calsequestrin 1 Monoclonal Antibody (ET7107-39)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Calsequestrin 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Calsequestrin 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Calsequestrin 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Calsequestrin 1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of C2C12 cells with Calsequestrin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Calsequestrin 1 antibody. Counter stained with hematoxylin.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Calsequestrin 1 Monoclonal Antibody (ET7107-39)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat skeletal muscle tissue, mouse skeletal muscle tissue, human fetal skeletal muscle tissue, C2C12.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB57-37

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

45 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Calsequestrin 1

SYNONYMS

Calmitin antibody; Calmitine antibody; Calsequestrin 1 (fast twitch, skeletal muscle) antibody; Calsequestrin antibody; Calsequestrin, cell antibody; Calsequestrin, skeletal muscle isoform antibody; Calsequestrin-1 antibody; CASQ antibody; Casq1 antibody; CASQ1_HUMAN antibody; PDIB1 antibody; skeletal muscle isoform antibody

SEQUENCE SIMILARITIES

Belongs to the calsequestrin family.

TISSUE SPECIFICITY

Expressed in myoblasts (at protein level).

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.

SUBCELLULAR LOCATION

Endoplasmic reticulum. Mitochondrion.

FUNCTION

Calsequestrin (CS, also known as CSQ) is the major calcium-binding protein of cardiac and skeletal muscle whose function is to sequester calcium in the lumen of the sarcoplasmic reticulum (SR). In mammals, there are two forms of this protein, calsequestrin 1 and calsequestrin 2, which encode fast-twitch skeletal muscle and cardiac calsequestrin, respectively. Calsequestrin 1, also known as Calmitin, is located in the terminal cisternae luminal spaces of the SR of fast skeletal muscle cells. Calsequestrin 2 is present in terminal cisternae luminal spaces of the SR of both cardiac and slow skeletal muscle cells. In addition, calsequestrin regulates the ryanodine receptor signaling (RyR) through Triadin and Junctin.