PRODUCT CODE: ET1704-90

Recombinant Calpain 2 Monoclonal Antibody (ET1704-90)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Calpain 2 on Hela cell (1) and rat skin tissue (2) lysate using anti-Calpain 2 antibody at 1/1,000 dilution.
  • Western blot analysis of Calpain 2 on Hela cell (1) and rat skin tissue (2) lysate using anti-Calpain 2 antibody at 1/1,000 dilution.
  • ICC staining Calpain 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Calpain 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calpain 2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Calpain 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of Calpain 2 on Hela cell (1) and rat skin tissue (2) lysate using anti-Calpain 2 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Calpain 2 Monoclonal Antibody (ET1704-90)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, rat skin tissue lysate, human lung tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA43-41

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

80 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Calpain 2

SYNONYMS

Calcium activated neutral proteinase 2 antibody; Calcium activated neutral proteinase antibody; Calcium-activated neutral protease 2, catalytic subunit antibody; Calcium-activated neutral proteinase 2 antibody; CALP80 antibody; Calpain 2 (m/II) large subunit antibody; Calpain 2 catalytic subunit antibody; Calpain 2 large catalytic subunit antibody; Calpain 2 large subunit antibody; Calpain 2, large [catalytic] subunit antibody; Calpain large polypeptide L2 antibody; Calpain M type antibody; Calpain M-type antibody; Calpain-2 catalytic subunit antibody; Calpain-2 large subunit antibody; Calpain2 antibody; CAN2_HUMAN antibody; CANP 2 antibody; CANP L2 antibody; CANP2 antibody; CANPL 2 antibody; CANPL2 antibody; CANPml antibody; Capa2 antibody; CAPN 2 antibody; CAPN2 antibody; FLJ39928 antibody; M calpain antibody; M calpin antibody; M type antibody; M-calpain antibody; mCANP antibody; Millimolar calpain antibody; Millimolar-calpain antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C2 family.

TISSUE SPECIFICITY

Ubiquitous.

SUBCELLULAR LOCATION

Cytoplasm. Cell membrane.

FUNCTION

Calpain, an intracellular calcium-dependent protease that cleaves cytoskeletal and submembranous proteins, is thought to play a role in cytoskeletal reorganization and muscle protein degradation. Calpain exists as a heterodimer composed of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpastatin regulates calpain by inhibiting both the proteolytic activity of calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing.