PRODUCT CODE: ET1704-02

Recombinant Calcineurin A Monoclonal Antibody (ET1704-02)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Calcineurin A on different cells lysates using anti-Calcineurin A antibody at 1/500 dilution.<br />
Positive control: <br />
 Lane 1: Hlea <br />
 Lane 2: 293 <br />
 Lane 3: A431
  • Western blot analysis of Calcineurin A on different cells lysates using anti-Calcineurin A antibody at 1/500 dilution.<br />
Positive control: <br />
 Lane 1: Hlea <br />
 Lane 2: 293 <br />
 Lane 3: A431
  • Flow cytometric analysis of Hela cells with Calcineurin A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Calcineurin A on different cells lysates using anti-Calcineurin A antibody at 1/500 dilution.
Positive control:
Lane 1: Hlea
Lane 2: 293
Lane 3: A431

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Calcineurin A Monoclonal Antibody (ET1704-02)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, 293, A431.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA64-11

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

59 kD

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

GENE NAME

PPP3CA

SYNONYMS

PPP3CA

SEQUENCE SIMILARITIES

Belongs to the PPP phosphatase family. PP-2B subfamily.

TISSUE SPECIFICITY

Expressed in keratinocytes (at protein level).

SUBCELLULAR LOCATION

Cytoplasm. Cell membrane; Peripheral membrane protein. Cell membrane, sarcolemma. Note=Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle (By similarity). Recruited to the cell membrane by scaffold protein AKAP5 following L-type Ca(2+)-channel activation.

FUNCTION

Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals. Many of the substrates contain a PxIxIT motif and/or a LxVP motif. In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation. In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion. Dephosphorylates heat shock protein HSPB1 (By similarity). Dephosphorylates and activates transcription factor NFATC1. In response to increased Ca(2+) levels, regulates NFAT-mediated transcription probably by dephosphorylating NFAT and promoting its nuclear translocation. Dephosphorylates and inactivates transcription factor ELK1. Dephosphorylates DARPP32. May dephosphorylate CRTC2 at 'Ser-171' resulting in CRTC2 dissociation from 14-3-3 proteins.