PRODUCT CODE: ET1702-54

Recombinant Calbindin Monoclonal Antibody (ET1702-54)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Calbindin on different lysates using anti-Calbindin antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Rat brain <br />
 Lane 2: Mouse kidney
  • Western blot analysis of Calbindin on different lysates using anti-Calbindin antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Rat brain <br />
 Lane 2: Mouse kidney
  • ICC staining Calbindin in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Calbindin in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Calbindin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calbindin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Calbindin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Calbindin antibody. Counter stained with hematoxylin.
Western blot analysis of Calbindin on different lysates using anti-Calbindin antibody at 1/1,000 dilution.
Positive control:
Lane 1: Rat brain
Lane 2: Mouse kidney

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Calbindin Monoclonal Antibody (ET1702-54)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-12, 293T, rat brain tissue, human kidney tissue, mouse kidney tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF05-01

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

28 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Calbindin

SYNONYMS

avian-type antibody; CAB27 antibody; CALB 1 antibody; CALB antibody; CALB1 antibody; CALB1_HUMAN antibody; Calbindin 1 28kDa antibody; Calbindin antibody; Calbindin D28 antibody; D 28K antibody; D-28K antibody; D28K antibody; OTTHUMP00000166027 antibody; OTTHUMP00000225441 antibody; RTVL H protein antibody; Vitamin D dependent calcium binding protein antibody; Vitamin D dependent calcium binding protein avian type antibody; Vitamin D-dependent calcium-binding protein antibody

SEQUENCE SIMILARITIES

Belongs to the calbindin family.

SUBCELLULAR LOCATION

Cytosol, Nucleus.

FUNCTION

The family of EF-hand type Ca2+-binding proteins includes Calbindin D28K, Calbindin D9K, S-100 α and β, Calgranulin A (also designated MRP8), Calgranulin B (also designated MRP14), Calgranulin C and the Parvalbumin family members, including Parvalbumin α and Parvalbumin β (also designated oncomodulin). Calbindin D28K, also known as calbindin, CALB1, D-28K or vitamin D-dependent calcium-binding protein, is a 261 amino acid protein with six EF-hand domains, four of which are active calcium-binding domains. Expressed in brain, ovary, uterus, testis, pancreas, liver, kidney and intestine, Calbindin D28K acts as a calcium-buffering agent and alters the activity of the plasma membrane ATPase. In neuronal cells, Calbindin D28K modulates calcium channel activity, calcium transients and intrinsic neuronal firing activity. Also, Calbindin D28K has been implicated to play a role in apoptosis and microtubule function.