PRODUCT CODE: ET1702-54

Recombinant Calbindin Monoclonal Antibody (ET1702-54)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Calbindin on different lysates using anti-Calbindin antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Rat brain <br />
Lane 2: Mouse kidney
  • Western blot analysis of Calbindin on different lysates using anti-Calbindin antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Rat brain <br />
Lane 2: Mouse kidney
  • ICC staining Calbindin in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Calbindin in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Calbindin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calbindin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Calbindin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Calbindin antibody. Counter stained with hematoxylin.
Western blot analysis of Calbindin on different lysates using anti-Calbindin antibody at 1/1,000 dilution.
Positive control:
Lane 1: Rat brain
Lane 2: Mouse kidney

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Calbindin Monoclonal Antibody (ET1702-54)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-12, 293T, rat brain tissue, human kidney tissue, mouse kidney tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF05-01

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

28 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Calbindin

GENE NAME

CALB1

SYNONYMS

CALB1

SEQUENCE SIMILARITIES

Belongs to the calbindin family.

FUNCTION

Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.