PRODUCT CODE: ET1705-10

Recombinant C4 Monoclonal Antibody (ET1705-10)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of C4 on human liver tissue lysate using anti-C4 antibody at 1/1,000 dilution.
  • Western blot analysis of C4 on human liver tissue lysate using anti-C4 antibody at 1/1,000 dilution.
  • ICC staining C4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-C4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-C4 antibody. Counter stained with hematoxylin.
Western blot analysis of C4 on human liver tissue lysate using anti-C4 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant C4 Monoclonal Antibody (ET1705-10)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM88-13

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

193 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:200-1:500

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

C4

SYNONYMS

Acidic C4 antibody; Acidic complement C4 antibody; Basic C4 antibody; Basic complement C4 antibody; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody; C4, Chido form antibody; C4, Rodgers from antibody; C4A anaphylatoxin antibody; C4A antibody; C4A2 antibody; C4A3 antibody; C4A4 antibody; C4A6 antibody; C4AD antibody; C4B antibody; C4B_2 antibody; C4B1 antibody; C4B12 antibody; C4B2 antibody; C4B3 antibody; C4BD antibody; C4F antibody; C4S antibody; CH antibody; Chido form of C4 antibody; CO4 antibody; CO4A_HUMAN antibody; Complement C4 gamma chain antibody; complement C4-A antibody; complement C4-B antibody; complement C4-B-like antibody; complement C4B1a antibody; Complement component 4A (Rodgers blood group) antibody; Complement component 4A antibody; complement component 4B (Chido blood group), copy 2 antibody; Complement component 4B (Childo blood group) antibody; Complement component 4B antibody; Complement component 4F antibody; Complement component 4S antibody; CPAMD2 antibody; CPAMD3 antibody; RG antibody; Rodgers form of C4 antibody

TISSUE SPECIFICITY

Complement component C4 is expressed at highest levels in the liver, at moderate levels in the adrenal cortex, adrenal medulla, thyroid gland, and the kidney, and at lowest levels in the heart, ovary, small intestine, thymus, pancreas and spleen. The extra-hepatic sites of expression may be important for the local protection and inflammatory response.

POST-TRANSLATIONAL MODIFICATION

Prior to secretion, the single-chain precursor is enzymatically cleaved to yield non-identical chains alpha, beta and gamma. During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase. The proteolytic cleavages often are incomplete so that many structural forms can be found in plasma.; N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

SUBCELLULAR LOCATION

Cell junction, Cell projection, Secreted, Synapse.

FUNCTION

The complement component proteins C3, C4 and C5 are potent anaphylatoxins that are released during complement activation, a system of ligand-surface protein interactions specific to cells of hematopoietic lineage. These proteins belong to the α2-macroglobulin family, but retain distinctive features including an anaphylatoxin domain and a netrin (NTR) domain. The human C4 gene is polymorphic at two loci, C4A and C4B, mapping to chromosome 6p21.32. C4A expresses the Rodgers (Rg) blood group Ag, while C4B expresses the Chido (Ch) blood group Ag. C4 is expressed as a precursor that is cleaved into α, β and γ chains, all of which are non-identical cleavage products. The α chain of C4 may be cleaved to produce an acidic isotype, C4a, which reacts with amino groups, and a basic isotype, C4b, which reacts with hydroxyl groups. Deficiency in the C4 gene is associated with autoimmune or immune complex disorders, such as systemic lupus erythematosus.