PRODUCT CODE: ET1706-46

Recombinant BST2 Monoclonal Antibody (ET1706-46)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

-
+
Western blot analysis of BST2 on Hela (1) and SiHa (2) cell lysate using anti-BST2 antibody at 1/500 dilution.
  • Western blot analysis of BST2 on Hela (1) and SiHa (2) cell lysate using anti-BST2 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-BST2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-BST2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with BST2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of BST2 on Hela (1) and SiHa (2) cell lysate using anti-BST2 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant BST2 Monoclonal Antibody (ET1706-46)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, SiHa, human colon cancer tissue, human kidney tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU11-93

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

40 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

BST2

SYNONYMS

Bone marrow stromal antigen 2 antibody; Bone marrow stromal cell antigen 2 antibody; Bone marrow stromal cell antigen antibody; BST 2 antibody; BST-2 antibody; BST2 antibody; BST2_HUMAN antibody; CD 317 antibody; CD317 antibody; CD317 antigen antibody; HM1.24 antigen antibody; NPC A 7 antibody; Tetherin antibody

SEQUENCE SIMILARITIES

Belongs to the tetherin family.

TISSUE SPECIFICITY

Predominantly expressed in liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal muscle and brain. Overexpressed in multiple myeloma cells. Highly expressed during B-cell development, from pro-B precursors to plasma cells. Highly expressed on T-cells, monocytes, NK cells and dendritic cells (at protein level).

POST-TRANSLATIONAL MODIFICATION

Monoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation.; The GPI anchor is essential for its antiviral activity.

SUBCELLULAR LOCATION

Golgi apparatus. Cell membrane.

FUNCTION

May be involved in the sorting of secreted proteins. May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. May play a role in B-cell activation in rheumatoid arthritis.