PRODUCT CODE: ET1611-6

Recombinant BPI Monoclonal Antibody (ET1611-6)

  • Zebrafish
  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Zebrafish

Western blot analysis of BPI on THP-1 cells lysates using anti-BPI antibody at 1/1,000 dilution.
  • Western blot analysis of BPI on THP-1 cells lysates using anti-BPI antibody at 1/1,000 dilution.
  • Flow cytometric analysis of Jurkat cells with BPI antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of BPI on THP-1 cells lysates using anti-BPI antibody at 1/1,000 dilution.

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant BPI Monoclonal Antibody (ET1611-6)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

THP-1.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN06-63

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

57 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Bactericidal permeability-increasing protein

GENE NAME

BPI

SYNONYMS

BPI

SEQUENCE SIMILARITIES

Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

TISSUE SPECIFICITY

Restricted to cells of the myeloid series.

SUBCELLULAR LOCATION

Secreted. Cytoplasmic granule membrane. Note=Membrane-associated in polymorphonuclear Leukocytes (PMN) granules.

FUNCTION

The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa.