PRODUCT CODE: ET1705-5

Recombinant BMAL1 Monoclonal Antibody (ET1705-5)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of BMAL1 on different lysates using anti-BMAL1 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
  Lane 1: NIH/3T3 <br />
  Lane 2: Rat brain <br />
  Lane 3: Mouse spleen <br />
  Lane 4: Hela
  • Western blot analysis of BMAL1 on different lysates using anti-BMAL1 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
  Lane 1: NIH/3T3 <br />
  Lane 2: Rat brain <br />
  Lane 3: Mouse spleen <br />
  Lane 4: Hela
  • ICC staining BMAL1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining BMAL1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of BMAL1 on different lysates using anti-BMAL1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: NIH/3T3
Lane 2: Rat brain
Lane 3: Mouse spleen
Lane 4: Hela

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant BMAL1 Monoclonal Antibody (ET1705-5)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela cell lysate, NIH/3T3 cell lysate, rat brain tissue lysate, mouse spleen tissue lysate, Hela, SH-SY5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM17-34

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

68 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

BMAL1

SYNONYMS

ARNT like protein 1 brain and muscle antibody; Arntl antibody; Aryl hydrocarbon receptor nuclear translocator like antibody; Aryl hydrocarbon receptor nuclear translocator like protein 1 antibody; Aryl hydrocarbon receptor nuclear translocator-like protein 1 antibody; Basic helix loop helix PAS orphan MOP3 antibody; Basic helix loop helix PAS protein MOP3 antibody; Basic-helix-loop-helix-PAS protein MOP3 antibody; bHLH PAS protein JAP3 antibody; bHLH-PAS protein JAP3 antibody; bHLHe5 antibody; BMAL 1 antibody; BMAL1_HUMAN antibody; BMAL1c antibody; Brain and muscle ARNT like 1 antibody; Brain and muscle ARNT-like 1 antibody; CG8727 PA antibody; Class E basic helix-loop-helix protein 5 antibody; cycle antibody; JAP 3 antibody; JAP3 antibody; Member of PAS protein 3 antibody; Member of PAS superfamily 3 antibody; MGC47515 antibody; MOP 3 antibody; MOP3 antibody; PAS domain-containing protein 3 antibody; PASD 3 antibody; PASD3 antibody; TIC antibody

TISSUE SPECIFICITY

Hair follicles (at protein level). Highly expressed in the adult brain, skeletal muscle and heart.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated, leading to its proteasomal degradation. Deubiquitinated by USP9X.; O-glycosylated; contains O-GlcNAc. O-glycosylation by OGT prevents protein degradation by inhibiting ubiquitination. It also stabilizes the CLOCK-ARNTL/BMAL1 heterodimer thereby increasing CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2/3 and CRY1/2.; Acetylated on Lys-538 upon dimerization with CLOCK. Acetylation facilitates CRY1-mediated repression. Deacetylated by SIRT1, which may result in decreased protein stability.; Phosphorylated upon dimerization with CLOCK. Phosphorylation enhances the transcriptional activity, alters the subcellular localization and decreases the stability of the CLOCK-ARNTL/BMAL1 heterodimer by promoting its degradation. Phosphorylation shows circadian variations in the liver with a peak between CT10 to CT14. Phosphorylation at Ser-90 by CK2 is essential for its nuclear localization, its interaction with CLOCK and controls CLOCK nuclear entry (By similarity). Dephosphorylation at Ser-78 is important for dimerization with CLOCK and transcriptional activity.; Sumoylated on Lys-259 upon dimerization with CLOCK. Predominantly conjugated to poly-SUMO2/3 rather than SUMO1 and the level of these conjugates undergo rhythmic variation, peaking at CT9-CT12. Sumoylation localizes it exclusively to the PML body and promotes its ubiquitination in the PML body, ubiquitin-dependent proteasomal degradation and the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer.; Undergoes lysosome-mediated degradation in a time-dependent manner in the liver.

SUBCELLULAR LOCATION

Nucleus, PML body, Cytoplasm.

FUNCTION

Transcriptional activator which forms a core component of the circadian clock. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. ARNTL/BMAL1 positively regulates myogenesis and negatively regulates adipogenesis via the transcriptional control of the genes of the canonical Wnt signaling pathway.