Positive control:
Lane 1: Raji cell lysate
Lane 2: Jurkat cell lysate
Lane 3: A431 cell lysate
Applications
-
WB
-
ICC
-
IHC-P
-
IP
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Bim Monoclonal Antibody (ET1608-14)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Raji cell lysate, Jurkat cell lysate, A431 cell lysate, Hela, A431, HepG2, human breast carcinoma tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SU0318
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
22/16/13 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC
-
1:50-1:200
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
Bim
SYNONYMS
BCL2 like 11 antibody; B2L11_HUMAN antibody; BAM antibody; Bcl 2 interacting protein Bim antibody; Bcl 2 related ovarian death agonist antibody; Bcl-2-like protein 11 antibody; BCL2 interacting mediator of cell death antibody; BCL2 like 11 (apoptosis facilitator) antibody; BCL2 like protein 11 antibody; Bcl2-interacting mediator of cell death antibody; Bcl2-L-11 antibody; Bcl2l11 antibody; BIM alpha6 antibody; BIM antibody; BIM beta6 antibody; BIM beta7 antibody; BimEL antibody; BimL antibody; BOD antibody
SEQUENCE SIMILARITIES
Belongs to the Bcl-2 family.
TISSUE SPECIFICITY
Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are widely expressed with tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Ser-69 by MAPK1/MAPK3 leads to interaction with TRIM2 and polyubiquitination, followed by proteasomal degradation. Deubiquitination catalyzed by USP27X stabilizes the protein (By similarity).; Ubiquitination by TRIM2 following phosphorylation by MAPK1/MAPK3 leads to proteasomal degradation. Conversely, deubiquitination catalyzed by USP27X stabilizes the protein.
SUBCELLULAR LOCATION
Endomembrane system, Mitochondrion.
FUNCTION
Pro-apototic Bcl-2 family members promote cell death by neutralizing their anti-apoptotic relatives, which otherwise maintain cell viability by regulating caspase activity. Bim belongs to the BH3-only subgroup of Bcl-2 related proteins, and exists in three distinct isoforms, BimS (short), BimL (long) and BimEL (extra long). ERK1/2 phosphorylates BimEL, resulting in rapid degradation of the isoform via the proteasome pathway. At least three sites for ERK1/2 phosphorylation exist on BimEL, whereas ERK1/2 does not effect BimS and BimL, implying a unique role for BimEL in cell survival signaling.