PRODUCT CODE: ET7107-48

Recombinant Biglycan Monoclonal Antibody (ET7107-48)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Biglycan on mouse heart and human skin tissue lysates using anti-Biglycan antibody at 1/500 dilution.
  • Western blot analysis of Biglycan on mouse heart and human skin tissue lysates using anti-Biglycan antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Biglycan antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Biglycan antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with Biglycan antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Biglycan on mouse heart and human skin tissue lysates using anti-Biglycan antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Biglycan Monoclonal Antibody (ET7107-48)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2, human kidney tissue, mouse heart tissue, human skin tissue lysates.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB71-31

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

42 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Biglycan

SYNONYMS

BGN antibody; Biglycan antibody; Biglycan proteoglycan antibody; Bone/cartilage proteoglycan I antibody; Dermatan sulphate proteoglycan I antibody; DSPG1 antibody; PG S1 antibody; PG-S1 antibody; PGI antibody; PGS1_HUMAN antibody; SEMDX antibody; SLRR1A antibody; Small leucine rich protein 1A antibody

SEQUENCE SIMILARITIES

Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

TISSUE SPECIFICITY

Found in several connective tissues, especially in articular cartilages.

POST-TRANSLATIONAL MODIFICATION

The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate.

SUBCELLULAR LOCATION

Extracellular matrix. Secreted.

FUNCTION

Biglycan, a class I small leucine rich proteoglycan (SLRP) present in the extracellular matrix, influences bone cell differentiation and proliferation. Biglycan contains two chondroitin sulfate glucosaminoglycan (GAG) chains attached near its amino terminus, whereas a closely related SLRP, decorin, contains only one. Biglycan deficient specimens possess diminished capacity to produce bone cells precursors, a lessened response to TGFβ, reduced collagen synthesis, and increased apoptosis. Patients with rheumatoid arthritis express increased immunity to biglycan whereas osteoarthritis patients do not, suggesting that higher immunity to SLRPs may play a role in the pathogenesis of inflammatory rheumatic diseases.