Positive control:
Lane 1: A431 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: mouse brain tissue lysate
Lane 5: Hela cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: PC-12 cell lysate
Applications
-
WB
-
ICC
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant beta Tubulin Monoclonal Antibody (ET1602-4)
Immunogen
Synthetic peptide within human beta tubulin aa 310-350.
Host
Rabbit
Positive Control
A431 cell lysate, MCF-7 cell lysate, SH-SY5Y cell lysate, mouse brain tissue lysate, Hela cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Hela, CRC, N2A, PC-12, SH-SY5Y, human colon carcinoma tissue, human kidney tissue, mouse colon tissue, mouse brain tissue, mouse kidney tissue, NIH/3T3.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SR25-04
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
50 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:5,000
-
ICC
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
beta Tubulin
SYNONYMS
Beta 4 tubulin antibody; Beta 5 tubulin antibody; BetaTubulin antibody; TBB5_HUMAN antibody; TUBB antibody; TUBB2 antibody; TUBB2A antibody; TUBB5 antibody; tubulin beta 2A antibody; Tubulin beta chain antibody; Tubulin beta-5 chain antibody
SEQUENCE SIMILARITIES
Belongs to the tubulin family.
TISSUE SPECIFICITY
Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
POST-TRANSLATIONAL MODIFICATION
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).; Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
SUBCELLULAR LOCATION
Cytoplasm
FUNCTION
Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP.