PRODUCT CODE: ET7106-74

Recombinant BDKRB2 Monoclonal Antibody (ET7106-74)

  • Recombinant

Applications

  • WB

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

Western blot analysis of BDKRB2 on MCF-7 cell lysate using anti-BDKRB2 antibody at 1/500 dilution.
  • Western blot analysis of BDKRB2 on MCF-7 cell lysate using anti-BDKRB2 antibody at 1/500 dilution.
  • Flow cytometric analysis of SH-SY5Y cells with BDKRB2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of BDKRB2 on MCF-7 cell lysate using anti-BDKRB2 antibody at 1/500 dilution.

Applications

  • WB

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant BDKRB2 Monoclonal Antibody (ET7106-74)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7, SH-SY5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU39-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

80 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

BDKRB2

SYNONYMS

B2 antibody; B2 bradykinin receptor antibody; B2BKR antibody; B2BRA antibody; B2R antibody; BDKR B2 antibody; BDKRB 2 antibody; BDKRB2 antibody; BK 2 antibody; BK 2 receptor antibody; BK R2 antibody; BK-2 receptor antibody; BK2 antibody; BK2 receptor antibody; BK2R antibody; BKR 2 antibody; BKR2 antibody; BKRB2_HUMAN antibody; BR B2 antibody; Bradykinin receptor B2 antibody; Bradykinin receptor beta 2 antibody; BRB 2 antibody; BRB2 antibody; DKFZp686O088 antibody; Kinin B2 antibody

SEQUENCE SIMILARITIES

Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB2 sub-subfamily.

TISSUE SPECIFICITY

Ubiquitous. Widespread in normal smooth muscle tissue and neurons.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

Kinins are important biologically active peptides that mediate cardiovascular homeostasis, inflammation and nociception. Bradykinin, the major effector peptide of the kallikrein-kinin system, is regulated by angiotensin-converting enzyme (ACE), which degrades the peptide. Bradykinin normally exerts its effects through the activation of two seven transmembrane G-protein coupled receptors, named B1 and B2. The B2 receptor is constitutively expressed and preferentially binds full length bradykinin. Deletion of the B2 receptor leads to salt-sensitive hypertension and altered nociception in mice. The B1 receptor binds to derivatives of bradykinin and kallidin, which are produced by carboxypeptidase action to generate the products des-Arg9-bradykinin and des-Arg10-kallidin, respectively. The expression of the B1 receptor is inducible by inflammatory mediators, such as bacterial lipopolysaccharide (LPS) and cytokines. The B1 and B2 receptors represent potential therapeutic targets for treatment of inflammatory disorders and cardiovascular diseases.