PRODUCT CODE: ET1611-79

Recombinant Bcl10 Monoclonal Antibody (ET1611-79)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

Western blot analysis of Bcl10 on different cell lysates using anti-Bcl10 antibody at 1/500 dilution.<br />
Positive control: <br />
Lane 1: HUVEC<br />
Lane 2:SW480
  • Western blot analysis of Bcl10 on different cell lysates using anti-Bcl10 antibody at 1/500 dilution.<br />
Positive control: <br />
Lane 1: HUVEC<br />
Lane 2:SW480
  • ICC staining Bcl10 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Bcl10 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bcl10 antibody. Counter stained with hematoxylin.
Western blot analysis of Bcl10 on different cell lysates using anti-Bcl10 antibody at 1/500 dilution.
Positive control:
Lane 1: HUVEC
Lane 2:SW480

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Bcl10 Monoclonal Antibody (ET1611-79)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SW480, MCF-7, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN74-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

31 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

B-cell lymphoma/leukemia 10

GENE NAME

BCL10

SYNONYMS

Bcl-10, hCLAP, CIPER, cCARMEN, c-E10, mE10, BCL10

TISSUE SPECIFICITY

Ubiquitous.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2. Phosphorylated by IKBKB/IKKB.

SUBCELLULAR LOCATION

Cytoplasm, perinuclear region. Membrane raft. Note=Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.

FUNCTION

Involved in adaptive immune response. Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.