PRODUCT CODE: ET1704-73

Recombinant Bag3 Monoclonal Antibody (ET1704-73)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

Western blot analysis of Bag3 on Hela (1) and MCF-7 (2) cell using anti-Bag3 antibody at 1/1,000 dilution.
  • Western blot analysis of Bag3 on Hela (1) and MCF-7 (2) cell using anti-Bag3 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Bag3 antibody. Counter stained with hematoxylin.
Western blot analysis of Bag3 on Hela (1) and MCF-7 (2) cell using anti-Bag3 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Bag3 Monoclonal Antibody (ET1704-73)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, MCF-7, human fetal skeletal muscle tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA90-53

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

75 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Bag3

SYNONYMS

BAG 3 antibody; BAG family molecular chaperone regulator 3 antibody; BAG-3 antibody; Bag3 antibody; BAG3_HUMAN antibody; Bcl 2 binding protein antibody; Bcl-2-associated athanogene 3 antibody; Bcl-2-binding protein Bis antibody; BCL2 associated athanogene 3 antibody; BCL2 binding athanogene 3 antibody; BIS antibody; CAIR 1 antibody; Docking protein CAIR 1 antibody; Docking protein CAIR-1 antibody; MFM6 antibody

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

The Bag family of proteins are characterized by the presence of a 45 amino acid Bag domain through which they bind with high affinity to the ATPase domain of HSP 70, thereby negatively regulating HSP 70 chaperone activity. Bag-3 (Bcl-2-associated athanogene 3), also known as BIS or CAIR-1, is a 575 amino acid protein that contains one C-terminal Bag domain and two N-terminal WW domains. Like other members of the Bag family, Bag-3 functions to inhibit the chaperone activity of HSP 70, specifically by promoting the release of HSP 70-bound substrates. Additionally, Bag-3 exhibits anti-apoptotic activity via cell cycle control, suggesting a possible role for Bag-3 in tumor progression. The gene encoding Bag-3 maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome.