PRODUCT CODE: ET1608-36

Recombinant B Raf Monoclonal Antibody (ET1608-36)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-B Raf antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-36, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-B Raf antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-36, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-B Raf antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-36, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-B Raf antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-36, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-B Raf antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-36, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-B Raf antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-36, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of B Raf was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1608-36, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-B Raf antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-36, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications

  • WB

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant B Raf Monoclonal Antibody (ET1608-36)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat brain tissue, human placenta tissue, human pancreas tissue, mouse fallopian tube tissue, mouse brain tissue, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SU34-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

85 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • FC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

B Raf

SYNONYMS

FLJ95109 antibody; 94 kDa B raf protein antibody; B raf 1 antibody; B Raf proto oncogene serine threonine protein kinase antibody; B Raf proto oncogene, serine/threonine kinase antibody; B RAF1 antibody; B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody; BRAF 1 antibody; BRAF antibody; BRAF_HUMAN antibody; BRAF1 antibody; cRmil antibody; MGC126806 antibody; MGC138284 antibody; Murine sarcoma viral (v-raf) oncogene homolog B1 antibody; Murine sarcoma viral v raf oncogene homolog B1 antibody; NS7 antibody; Oncogen BRAF antibody; oncogene BRAF1 antibody; p94 antibody; Proto-oncogene B-Raf antibody; Proto-oncogene c-Rmil antibody; RAFB 1 antibody; RAFB1 antibody; RMIL antibody; Serine/threonine-protein kinase B-raf antibody; v raf murine sarcoma viral oncogene homolog B antibody; v raf murine sarcoma viral oncogene homolog B1 antibody; v-Raf murine sarcoma viral oncogene homolog B1 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.

TISSUE SPECIFICITY

Brain and testis.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-365 by SGK1 inhibits its activity.; Methylation at Arg-671 decreases stability and kinase activity.; Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm, Cell membrane.

FUNCTION

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.