PRODUCT CODE: ET1703-96

Recombinant Axin2 Monoclonal Antibody (ET1703-96)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Axin2 on SW480 cells lysates using anti-Axin2 antibody at 1/500 dilution.
  • Western blot analysis of Axin2 on SW480 cells lysates using anti-Axin2 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Axin2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Axin2 antibody. Counter stained with hematoxylin.
Western blot analysis of Axin2 on SW480 cells lysates using anti-Axin2 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Axin2 Monoclonal Antibody (ET1703-96)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SW480, mouse colon tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-30

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

94 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Axin-2

GENE NAME

AXIN2

SYNONYMS

Axil, AXIN2

TISSUE SPECIFICITY

Expressed in brain and lymphoblast.

POST-TRANSLATIONAL MODIFICATION

Probably phosphorylated by GSK3B and dephosphorylated by PP2A.; ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.; Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B.