PRODUCT CODE: ET1610-25

Recombinant Aurora B Monoclonal Antibody (ET1610-25)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

Western blot analysis of Aurora B on different lysates using anti-Aurora B antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: MCF-7
  • Western blot analysis of Aurora B on different lysates using anti-Aurora B antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: MCF-7
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Aurora B antibody. Counter stained with hematoxylin.
Western blot analysis of Aurora B on different lysates using anti-Aurora B antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: MCF-7

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Aurora B Monoclonal Antibody (ET1610-25)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7, Hela, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC55-08

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

39 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Aurora B

SYNONYMS

AIK2 antibody; AIM-1 antibody; AIM1 antibody; ARK-2 antibody; ARK2 antibody; AurB antibody; AURKB antibody; AURKB_HUMAN antibody; Aurora 1 antibody; Aurora and Ipl1 like midbody associated protein 1 antibody; Aurora kinase B antibody; Aurora related kinase 2 antibody; Aurora- and Ipl1-like midbody-associated protein 1 antibody; Aurora-B antibody; Aurora-related kinase 2 antibody; Aurora/IPL1 related kinase 2 antibody; Aurora/IPL1-related kinase 2 antibody; IPL1 antibody; PPP1R48 antibody; Protein phosphatase 1 regulatory subunit 48 antibody; Serine/theronine kinase 12 antibody; Serine/threonine protein kinase 12 antibody; Serine/threonine-protein kinase 12 antibody; Serine/threonine-protein kinase aurora-B antibody; STK-1 antibody; STK1 antibody; STK12 antibody; STK5 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.

TISSUE SPECIFICITY

High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.

POST-TRANSLATIONAL MODIFICATION

The phosphorylation of Thr-232 requires the binding to INCENP and occurs by means of an autophosphorylation mechanism. Thr-232 phosphorylation is indispensable for the AURKB kinase activity.; Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm, Chromosome.

FUNCTION

Aurora related kinase-1 (ARK-1, STK15, Aurora2, Aik1) and -2 (ARK-2, STK12, Aurora1) are centrosome-associated serine/ threonine kinases that regulate centrosome separation, bipolar spindle assembly, and chromosome segregation during mitosis. ARK-1 and -2 are expressed in the nucleus and localize to distinct portions of mitotic machinery such as the centrosome, spindle poles (ARK-1), and midbody (ARK-2) during mitosis. ARK-1 and -2 transcripts are present at high levels in human thymus and fetal liver. ARK-1 protein has elevated expression in colon carcinoma lines (HT-29, SNU-C2B, COLO 205, SW480, 837 and 948) and accumulates during metaphase in HeLa cells. ARK-2 protein levels are maximal during both S and G2/M phases, whereas ARK-1 protein is degraded after G2/M via the ubiquitin-proteasome pathway. ARK-2 has a unique genetic loci relative to ARK-1, suggesting that these two kinases, with oncogenic potential, have different roles in cell cycle progression.