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PRODUCT CODE: ET1703-29

Recombinant ATPB Monoclonal Antibody (ET1703-29)

  • Zebrafish
  • Recombinant

Applications

  • WB

  • IP

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

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Western blot analysis of ATPB on different cells lysates using anti-ATPB antibody at 1/500 dilution.<br /> Positive control: Line1: Hela Line2: HepG2 Line3: 293T
  • Western blot analysis of ATPB on different cells lysates using anti-ATPB antibody at 1/500 dilution.<br /> Positive control: Line1: Hela Line2: HepG2 Line3: 293T
  • ICC staining ATPB in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ATPB in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ATPB in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ATPB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ATPB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded huaman uterus tissue using anti-ATPB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ATPB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATPB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATPB antibody. Counter stained with hematoxylin.
  • Western blot analysis of ATPB on Zebrafish cells lysates using anti-ATPB antibody at 1/500 dilution.
Western blot analysis of ATPB on different cells lysates using anti-ATPB antibody at 1/500 dilution.
Positive control: Line1: Hela Line2: HepG2 Line3: 293T

Applications

  • WB

  • IP

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant ATPB Monoclonal Antibody (ET1703-29)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293T, HepG2, A431, Hela, human liver tissue, human kidney tissue, mouse uterus tissue, mouse liver muscle tissue, mouse brain tissue, mouse heart tissue, zebrafish tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-90

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

53 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

P06576

PROTEIN NAME

ATPB

SYNONYMS

ATP 5B antibody; ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody; ATP synthase subunit beta mitochondrial antibody; ATP synthase subunit beta, mitochondrial antibody; atp5b antibody; ATPB antibody; ATPB_HUMAN antibody; ATPMB antibody; ATPSB antibody; Epididymis secretory protein Li 271 antibody; HEL-S-271 antibody; Mitochondrial ATP synthase beta subunit antibody; Mitochondrial ATP Synthase Subunit Beta antibody; Mitochondrial ATP synthetase beta subunit antibody

SEQUENCE SIMILARITIES

Belongs to the ATPase alpha/beta chains family.

SUBCELLULAR LOCATION

Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.

FUNCTION

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.