PRODUCT CODE: ET1610-71

Recombinant ATG9A Monoclonal Antibody (ET1610-71)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

ICC staining ATG9A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ATG9A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ATG9A in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ATG9A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATG9A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATG9A antibody. Counter stained with hematoxylin.
ICC staining ATG9A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • ICC

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant ATG9A Monoclonal Antibody (ET1610-71)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2 cell lysates, Hela, 293, rat brain tissue, mouse brain tissue, mouse heart tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC67-05

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

94 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

ATG9A

SYNONYMS

APG9 autophagy 9-like 1 antibody; APG9 like 1 antibody; APG9-like 1 antibody; APG9L1 antibody; ATG9 antibody; ATG9 autophagy related 9 homolog A antibody; ATG9 autophagy related 9 homolog A (S. cerevisiae) antibody; ATG9A antibody; ATG9A_HUMAN antibody; Autophagy 9-like 1 protein antibody; Autophagy related protein 9A antibody; Autophagy-related protein 9A antibody; mATG9 antibody; MGD3208 antibody; OTTHUMP00000206046 antibody; OTTHUMP00000206048 antibody; OTTHUMP00000206049 antibody; OTTHUMP00000206062 antibody

SEQUENCE SIMILARITIES

Belongs to the ATG9 family.

SUBCELLULAR LOCATION

Cytoplasmic vesicle, Golgi apparatus, Late endosome membrane, Endoplasmic reticulum membrane.

FUNCTION

Autophagy, a process that results in the lysosomal-dependent degradation of cytosolic compartments, is carried out by the autophagosome, which is a double-membrane vesicle whose formation is catalyzed by several autophagy-related gene (Atg) proteins. Atg9a (autophagy-related protein 9A), also known as APG9-like 1, is a 839 amino acid, multi-pass membrane protein that localizes to the pre-autophagosomal structure (PAS). Isolation membranes are suggested to originate from the PAS, enwrapping cytoplasmic components to form a double membrane autophagosome, which then fuses with the vacuole. Ubiquitously expressed in human adult tissues, Atg9a cycles between the Golgi and endosomes and, with the autophagosome-specific marker LC3, plays a critical role in starvation-induced autophagosome formation. Three isoforms of Atg9a exist as a result of alternative splicing events.