PRODUCT CODE: ET7106-65

Recombinant Atg16L1 Monoclonal Antibody (ET7106-65)

  • Recombinant
  • Test-Size

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Atg16L1 on Hela and PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7106-65, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of Atg16L1 on Hela and PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7106-65, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Atg16L1 on Hela and PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7106-65, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Atg16L1 Monoclonal Antibody (ET7106-65)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela and PC-12 cell lysates.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU30-62

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

68/66 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-2,000

TARGET

UNIPROT #

PROTEIN NAME

Atg16L1

SYNONYMS

A16L1_HUMAN antibody; APG16 like 1 antibody; APG16-like 1 antibody; APG16L antibody; APG16L beta antibody; ATG16 autophagy related 16 like 1 antibody; ATG16 autophagy related 16-like 1 (S. cerevisiae) antibody; ATG16A antibody; ATG16L antibody; Atg16l1 antibody; Autophagy related protein 16 1 antibody; Autophagy-related protein 16-1 antibody; FLJ00045 antibody; FLJ10035 antibody; FLJ10828 antibody; FLJ22677 antibody; IBD10 antibody; OTTHUMP00000164391 antibody; OTTHUMP00000164393 antibody; OTTHUMP00000165876 antibody; OTTHUMP00000165877 antibody; WD repeat domain 30 antibody; WDR30 antibody

SEQUENCE SIMILARITIES

Belongs to the WD repeat ATG16 family.

POST-TRANSLATIONAL MODIFICATION

Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.; Phosphorylation at Ser-139 promotes association with the ATG12-ATG5 conjugate to form the ATG12-ATG5-ATG16L1 complex.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane. Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production. Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response. Plays a role in regulating morphology and function of Paneth cell.