PRODUCT CODE: ET1609-24

Recombinant ASH2L Monoclonal Antibody (ET1609-24)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of ASH2L on different lysates using anti-ASH2L antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: PC12 <br />
Lane 2: 293T
  • Western blot analysis of ASH2L on different lysates using anti-ASH2L antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: PC12 <br />
Lane 2: 293T
  • ICC staining ASH2L in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ASH2L in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ASH2L in CRC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASH2L antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-ASH2L antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-ASH2L antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-ASH2L antibody. Counter stained with hematoxylin.
Western blot analysis of ASH2L on different lysates using anti-ASH2L antibody at 1/1,000 dilution.
Positive control:
Lane 1: PC12
Lane 2: 293T

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant ASH2L Monoclonal Antibody (ET1609-24)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC12, MCF-7, CRC, mouse ovary tissue, mouse placenta tissue, mouse testis tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

ST47-01

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

69 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:200-1:500

  • IHC-P

  • 1:200-1:500

TARGET

UNIPROT #

PROTEIN NAME

Set1/Ash2 histone methyltransferase complex subunit ASH2

GENE NAME

ASH2L

SYNONYMS

ASH2L, ASH2L1

TISSUE SPECIFICITY

Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes.

POST-TRANSLATIONAL MODIFICATION

Both monomethylated and dimethylated on arginine residues in the C-terminus. Arg-296 is the major site. Methylation is not required for nuclear localization, nor for MLL complex integrity or maintenance of global histone H3K4me3 levels.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Transcriptional regulator. Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters. Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May play a role in hematopoiesis. In association with RBBP5 and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B.