PRODUCT CODE: ET1703-77

Recombinant Aromatase Monoclonal Antibody (ET1703-77)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Rat

Western blot analysis of Aromatase on different cells lysates using anti-Aromatase antibody at 1/500 dilution.<br />
Positive control:<br />
Lane 1: JAR<br />
Lane 2: Jurkat
  • Western blot analysis of Aromatase on different cells lysates using anti-Aromatase antibody at 1/500 dilution.<br />
Positive control:<br />
Lane 1: JAR<br />
Lane 2: Jurkat
Western blot analysis of Aromatase on different cells lysates using anti-Aromatase antibody at 1/500 dilution.
Positive control:
Lane 1: JAR
Lane 2: Jurkat

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Aromatase Monoclonal Antibody (ET1703-77)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

JAR, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-68

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

58 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Aromatase

GENE NAME

CYP19A1

SYNONYMS

CYP19A1

SEQUENCE SIMILARITIES

Belongs to the cytochrome P450 family.

TISSUE SPECIFICITY

Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated in vitro by PKA and PKG/PRKG1. These phosphorylations inhibit the catalytic activity as measured by estrone synthesis from androstenedione (36% decrease for PKA and 30% for PKG/PRKG1).

SUBCELLULAR LOCATION

Endoplasmic reticulum membrane; Multi-pass membrane protein; Multi-pass membrane protein.

FUNCTION

A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively. Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid. Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen. Also displays 2-hydroxylase activity toward estrone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).